Human Xenotropic and polytropic retrovirus receptor 1 ELISA Kit | XPR1 elisa kit
Human Xenotropic and polytropic retrovirus receptor 1 (XPR1) ELISA Kit
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Northern blot analysis detected a 4.5-kb XPR1 transcript in all tissues tested, with highest expression in pancreas, kidney, placenta, hematopoietic tissues, and heart, and lowest expression in skeletal muscle. Expression of XPR1 was greater in fetal liver than adult liver. A 9.5-kb XPR1 transcript was also detected in all tissues tested except liver and bone marrow.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XPR1 in samples. An antibody specific for XPR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a receptor for the xenotropic and polytropic classes of murine leukemia viruses. The encoded protein is involved in phosphate homeostasis by mediating phosphate export from the cell. Defects in this gene have been associated with idiopathic basal ganglia calcification-6. [provided by RefSeq, Jun 2016]
Uniprot Description
XPR1: May function in G-protein coupled signal transduction. Potential receptor for xenotropic and polytropic murine leukemia retroviruses. Belongs to the SYG1 (TC 2.A.94) family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 1q25.3
Cellular Component: cytoplasm; integral component of membrane; intrinsic component of plasma membrane
Molecular Function: efflux transmembrane transporter activity; G-protein coupled receptor activity; inositol hexakisphosphate binding; phosphate ion transmembrane transporter activity; receptor activity; transmembrane receptor activity; viral receptor activity
Biological Process: cellular phosphate ion homeostasis; entry of virus into host cell; G-protein coupled receptor signaling pathway; response to virus
Disease: Basal Ganglia Calcification, Idiopathic, 6
Research Articles on XPR1
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Product Notes
The Human XPR1 xpr1 (Catalog #AAA285826) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285826 ELISA Kit recognizes Human XPR1. It is sometimes possible for the material contained within the vial of "Xenotropic and polytropic retrovirus receptor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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