Mouse Xaa-Pro aminopeptidase 1 ELISA Kit | XPNPEP1 elisa kit
Mouse Xaa-Pro aminopeptidase 1 (XPNPEP1) ELISA Kit
Gene Names
Xpnpep1; sAMP; D230045I08Rik
Reactivity
Mouse
Synonyms
Xaa-Pro aminopeptidase 1; Mouse Xaa-Pro aminopeptidase 1 (XPNPEP1) ELISA Kit; XPNPEP1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse XPNPEP1. No significant cross-reactivity or interference between Mouse XPNPEP1 and analogues was observed.
Sequence Length
666
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for XPNPEP1 elisa kit
X-prolyl aminopeptidase is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. Because of its specificity toward proline, it has been suggested that X-prolyl aminopeptidase is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases.
XPNPEPL, that encodes a 623-amino acid protein exhibiting 44% sequence identity and 62% sequence similarity to pig kidney X-prolyl aminopeptidase and high sequence homology to proteins in S. pombe and S. cerevisiae.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XPNPEP1 in samples. An antibody specific for XPNPEP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPNPEP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPNPEP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPNPEP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
XPNPEPL, that encodes a 623-amino acid protein exhibiting 44% sequence identity and 62% sequence similarity to pig kidney X-prolyl aminopeptidase and high sequence homology to proteins in S. pombe and S. cerevisiae.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XPNPEP1 in samples. An antibody specific for XPNPEP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXPNPEP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XPNPEP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XPNPEP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,591 Da
NCBI Official Full Name
xaa-Pro aminopeptidase 1
NCBI Official Synonym Full Names
X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
NCBI Official Symbol
Xpnpep1
NCBI Official Synonym Symbols
sAMP; D230045I08Rik
NCBI Protein Information
xaa-Pro aminopeptidase 1
UniProt Protein Name
Xaa-Pro aminopeptidase 1
Protein Family
UniProt Gene Name
Xpnpep1
UniProt Synonym Gene Names
sAmp
Uniprot Description
XPNPEP1: Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Belongs to the peptidase M24B family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.11.9; Protease
Chromosomal Location of Human Ortholog: 19|19 D2
Cellular Component: cytoplasm; cytosol; extracellular exosome
Molecular Function: aminopeptidase activity; hydrolase activity; manganese ion binding; metal ion binding; metalloaminopeptidase activity; metallopeptidase activity; peptidase activity; protein homodimerization activity
Biological Process: bradykinin catabolic process; proteolysis
Research Articles on XPNPEP1
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Product Notes
The Mouse XPNPEP1 xpnpep1 (Catalog #AAA280734) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280734 ELISA Kit recognizes Mouse XPNPEP1. It is sometimes possible for the material contained within the vial of "Xaa-Pro aminopeptidase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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