XAF1 recombinant protein

XAF1, 1- 125aa, Human, His tag, E Coli

Cat. Num. AAA205540

• Gene Names: XAF1; BIRC4BP; XIAPAF1; HSXIAPAF1
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: XAF1; 1- 125aa; Human; His tag; E Coli; XIAP associated factor 1 isoform 1; BIRC4BP; HSXIAPAF1; XIAPAF1; XAF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEGD FSVCRNCKRH VVSANFTLHE AYCLRFLVLC PECEEPVPKE TMEEHCKLEH QQVGCTMCQQ SMQKSSLEFH KANECQERPV ECKFCKLDMQ LSKLELHESY CGSRTELCQG CGQFIMHRML A
• Sequence Length: 301

• Applicable Applications for XAF1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for XAF1 recombinant protein

XAF1 is a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human XAF1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for XAF1 recombinant protein

Apoptosis

References

Liston P., et al (2001). Nat. Cell Biol. 3:128-133; Leaman D.W., et al (2002). J. Biol. Chem. 277:28504-28511;

NCBI and Uniprot Product Information

• NCBI GI #: 40288191
• NCBI GeneID: 54739
• NCBI Accession #: NP_059993
• NCBI GenBank Nucleotide #: NM_017523.4
• Molecular Weight: 6 kDa (161aa) confirmed by MALDI-TOF
• NCBI Official Full Name: XIAP-associated factor 1 isoform 1
• NCBI Official Synonym Full Names: XIAP associated factor 1
• NCBI Official Symbol: XAF1
• NCBI Official Synonym Symbols: BIRC4BP; XIAPAF1; HSXIAPAF1
• NCBI Protein Information: XIAP-associated factor 1
• UniProt Protein Name: XIAP-associated factor 1
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XAF1
• UniProt Synonym Gene Names: BIRC4BP; XIAPAF1

NCBI Description

This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.