Human XIAP-associated factor 1 ELISA Kit | XAF1 elisa kit

Human XIAP-associated factor 1 (XAF1) ELISA Kit

Cat. Num. AAA285662

• Gene Names: XAF1; BIRC4BP; XIAPAF1; HSXIAPAF1
• Reactivity: Human
• Synonyms: XIAP-associated factor 1; Human XIAP-associated factor 1 (XAF1) ELISA Kit; BIRC4BP; HSXIAPAF1; XIAPAF1; BIRC4 binding protein; XAF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human XAF1. No significant cross-reactivity or interference between Human XAF1 and analogues was observed.
• Sequence Length: 301

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for XAF1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate XAF1 in samples. An antibody specific for XAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyXAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for XAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 40288191
• NCBI GeneID: 54739
• NCBI Accession #: NP_059993.2
• NCBI GenBank Nucleotide #: NM_017523.4
• UniProt Accession #: Q6GPH4; Q6MZE8; Q8N557; Q99982; A2T931; A2T932; A8K2L1; A8K9Y3; D3DTM6
• Molecular Weight: 34,543 Da
• NCBI Official Full Name: XIAP-associated factor 1 isoform 1
• NCBI Official Synonym Full Names: XIAP associated factor 1
• NCBI Official Symbol: XAF1
• NCBI Official Synonym Symbols: BIRC4BP; XIAPAF1; HSXIAPAF1
• NCBI Protein Information: XIAP-associated factor 1
• UniProt Protein Name: XIAP-associated factor 1
• Protein Family: XIAP-associated factor
• UniProt Gene Name: XAF1
• UniProt Synonym Gene Names: BIRC4BP; XIAPAF1

NCBI Description

This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytosol; mitochondrion; nucleus

Molecular Function: zinc ion binding

Biological Process: apoptosis