WW Domain Containing Oxidoreductase Recombinant Protein | WWOX recombinant protein

Recombinant Human WW Domain Containing Oxidoreductase

Cat. Num. AAA143535

• Gene Names: WWOX; FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• Purity: Greater than 85.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: WW Domain Containing Oxidoreductase; Recombinant Human WW Domain Containing Oxidoreductase; WWOX Human; WW Domain Containing Oxidoreductase Human Recombinant; FOR; WOX1; FRA16D; HHCMA56; PRO0128; SDR41C1; D16S432E; WWOX; WW domain-containing oxidoreductase; Fragile site FRA16D oxidoreductase; WWOX recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: The WWOX solution (1mg/ml) contains 20mM Tris pH-8, & 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAALRYAGLD DTDSEDELPP GWEERTTKDG WVYYANHTEE KTQWEHPKTG KRKRVAGDLP YGWEQETDEN GQVFFVDHIN KRTTYLDPRL AFTVDDNPTK PTTRQRYDGS TTAMEILQGR DFTGKVVVVT GANSGIGFET AKSFALHGAH VILACRNMAR ASEAVSRILE EWQQGAATTV YCAAVPELEG LGGMYFNNCC RCMPSPEAQS EETARTLWAL SERLIQERLG SQSG
• Sequence Length: 301

• Preparation and Storage: WWOX although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for WWOX recombinant protein

Description: WWOX Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 254 amino acids (1-234 a.a.) and having a molecular mass of 28.3 kDa.The WWOX is purified by proprietary chromatographic techniques.

Introduction: WWOX is a proapoptotic protein and a tumor suppressor protein. WWOX is found in all eukaryotes and involved in the regulation of a broad range of cellular functions such as protein degradation, transcription, and RNA splicing. WWOX functions synergistically with TP53/p53 to control genotoxic stress-induced cell death. WWOX takes part in tumor necrosis factor (TNF)-mediated cell death. Loss of WWOX expression is associated with pancreatobiliary cancers. Reduced expression levels of WWOX protein is associated with the pathogenesis of basal-like differentiation in breast cancer. Loss of WWOX expression is associated with extrahepatic cholangiocarcinoma. WWOX gene alteration is an early genetic alteration contributes to oral carcinogenesis. WWOX induces apoptosis and inhibits human hepatocellular carcinoma cell growth through a mechanism enhanced by JNK inhibition.

Product Categories/Family for WWOX recombinant protein

Enzymes; Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 635172870
• NCBI GeneID: 51741
• NCBI Accession #: NP_001278926.1
• NCBI GenBank Nucleotide #: NM_001291997.1
• UniProt Accession #: Q9NZC7; Q5MYT5; Q96KM3; Q96RF2; Q9BTT8; Q9NPC9; Q9NRF4; Q9NRF5; Q9NRF6; Q9NRK1; Q9NZC5; A8K323
• Molecular Weight: 23,868 Da
• NCBI Official Full Name: WW domain-containing oxidoreductase isoform 4
• NCBI Official Synonym Full Names: WW domain containing oxidoreductase
• NCBI Official Symbol: WWOX
• NCBI Official Synonym Symbols: FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
• NCBI Protein Information: WW domain-containing oxidoreductase; WW domain-containing protein WWOX; fragile site FRA16D oxidoreductase; short chain dehydrogenase/reductase family 41C, member 1
• UniProt Protein Name: WW domain-containing oxidoreductase
• Protein Family: WW domain-containing oxidoreductase
• UniProt Gene Name: WWOX
• UniProt Synonym Gene Names: FOR; WOX1
• UniProt Entry Name: WWOX_HUMAN

NCBI Description

This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) protein family. This gene spans the FRA16D common chromosomal fragile site and appears to function as a tumor suppressor gene. Expression of the encoded protein is able to induce apoptosis, while defects in this gene are associated with multiple types of cancer. Disruption of this gene is also associated with autosomal recessive spinocerebellar ataxia 12. Disruption of a similar gene in mouse results in impaired steroidogenesis, additionally suggesting a metabolic function for the protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

WWOX: an enzyme which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). Expressed at high levels in hormonally regulated tissues such as testis, ovary, and prostate. Its SRD domain suggest a role for this gene in steroid metabolism. Shown to be an essential mediator of tumor necrosis factor-alpha-induced apoptosis in the mouse. May play a similar role in the human. May function as a suppressor of tumor growth. Alternative splicing of this gene generates 7 isoforms.

Protein type: Oxidoreductase; Tumor suppressor; Apoptosis; EC 1.1.1.-

Chromosomal Location of Human Ortholog: 16q23

Cellular Component: Golgi apparatus; microvillus; mitochondrion; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein dimerization activity; protein binding; enzyme binding; oxidoreductase activity; cofactor binding; coenzyme binding

Biological Process: steroid metabolic process; osteoblast differentiation; negative regulation of Wnt receptor signaling pathway; Wnt receptor signaling pathway; skeletal morphogenesis; positive regulation of transcription from RNA polymerase II promoter

Disease: Epileptic Encephalopathy, Early Infantile, 28