WTAP recombinant protein

WTAP Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121052

• Purity: >70% as determined by SDS-PAGE
• Synonyms: WTAP; WTAP Protein; Human; Recombinant (GST Tag); Human WTAP Protein (GST Tag); Mum2 Protein; Wilms tumor 1 associated protein; WTAP recombinant protein

• Host: E Coli
• Purity/Purification: >70% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.15M NaCl, 0.5mM GSH, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg151

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human WTAP isoform 2 (Q15007-2)(Met 1-Arg 151) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for WTAP recombinant protein

Background: Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.

References

Fukusumi Y, et al. (2008) Wtap is required for differentiation of endoderm and mesoderm in the mouse embryo. Dev Dyn. 237(3): 618-29.Small TW, et al. (2007) Vascular biology and the sex of flies: regulation of vascular smooth muscle cell proliferation by wilms' tumor 1-associating protein. Trends Cardiovasc Med. 17(7): 230-4.Small TW, et al. (2006) Wilms' tumor 1-associating protein regulates the proliferation of vascular smooth muscle cells. Circ Res. 99(12): 1338-46.Rong Y, et al. (2006) Wilms' tumor 1 and signal transducers and activators of transcription 3 synergistically promote cell proliferation: a possible mechanism in sporadic Wilms' tumor. Cancer Res. 66(16): 8049-57.

NCBI and Uniprot Product Information

• NCBI GI #: 395455090
• NCBI GeneID: 9589
• NCBI Accession #: NP_001257460.1
• NCBI GenBank Nucleotide #: NM_001270531.1
• UniProt Accession #: Q15007; Q5TCL8; Q5TCL9; Q96T28; Q9BYJ7; Q9H4E2
• Molecular Weight: 44,244 Da
• NCBI Official Full Name: pre-mRNA-splicing regulator WTAP isoform 1
• NCBI Official Synonym Full Names: Wilms tumor 1 associated protein
• NCBI Official Symbol: WTAP
• NCBI Protein Information: pre-mRNA-splicing regulator WTAP; hFL(2)D; PNAS-132; WT1-associated protein; female-lethal(2)D homolog; wilms tumor 1-associating protein; Wilms' tumour 1-associating protein; putative pre-mRNA splicing regulator female-lethal(2D)
• UniProt Protein Name: Pre-mRNA-splicing regulator WTAP
• Protein Family: Pre-mRNA-splicing regulator
• UniProt Gene Name: WTAP
• UniProt Synonym Gene Names: KIAA0105; hFL(2)D
• UniProt Entry Name: FL2D_HUMAN

NCBI Description

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Spliceosome

Chromosomal Location of Human Ortholog: 6q25-q27

Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nuclear speck; nucleus

Biological Process: RNA splicing; cell cycle; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome