Typical Testing Data/Standard Curve (for reference only)
Human Neurofilament medium polypeptide (NEFM) ELISA Kit | NEFM elisa kit
Human Neurofilament medium polypeptide (NEFM) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: NEFM ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NEFM antibody and an NEFM-HRP conjugate. The assay sample and buffer are incubated together with NEFM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NEFM concentration since NEFM from samples and NEFM-HRP conjugate compete for the anti-NEFM antibody binding site. Since the number of sites is limited, as more sites are occupied by NEFM from the sample, fewer sites are left to bind NEFM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NEFM concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 8p21
Cellular Component: axon; neurofilament; neuromuscular junction
Molecular Function: protein binding; structural constituent of cytoskeleton; microtubule binding
Biological Process: neurofilament bundle assembly; axon cargo transport; regulation of axon diameter; microtubule cytoskeleton organization and biogenesis
Research Articles on NEFM
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Product Notes
The Human NEFM nefm (Catalog #AAA7226716) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7226716 ELISA Kit recognizes Human NEFM. It is sometimes possible for the material contained within the vial of "Neurofilament medium polypeptide (NEFM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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