WTAP recombinant protein

Recombinant Human WTAP Protein (GST tag)

Cat. Num. AAA2545600

• Gene Names: WTAP; Mum2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: WTAP; Recombinant Human WTAP Protein (GST tag); RP1-56L9.4; DKFZp686F20131; KIAA0105; MGC3925; WTAP recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.15M NaCl, 0.5mM GSH, pH 8.0
• Sequence Length: 396

• Application Notes: The recombinant human WTAP/GST chimera consists of 386 amino acids and migrates as an approxiamtely 45 kDa band as predicted in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for WTAP recombinant protein

Background: Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G (1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.

Description: A DNA sequence encoding the human WTAP isoform 2 (Q15007-2) (Met 1-Arg 151) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 395455090
• NCBI GeneID: 9589
• NCBI Accession #: NP_001257460.1
• NCBI GenBank Nucleotide #: NM_001270531.1
• UniProt Accession #: Q15007; Q5TCL8; Q5TCL9; Q96T28; Q9BYJ7; Q9H4E2
• Molecular Weight: 17,801 Da
• NCBI Official Full Name: pre-mRNA-splicing regulator WTAP isoform 1
• NCBI Official Synonym Full Names: Wilms tumor 1 associated protein
• NCBI Official Symbol: WTAP
• NCBI Official Synonym Symbols: Mum2
• NCBI Protein Information: pre-mRNA-splicing regulator WTAP
• UniProt Protein Name: Pre-mRNA-splicing regulator WTAP
• Protein Family: Pre-mRNA-splicing regulator
• UniProt Gene Name: WTAP
• UniProt Synonym Gene Names: KIAA0105; hFL(2)D

NCBI Description

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Spliceosome

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: nuclear membrane; nuclear speck; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: gene expression; regulation of alternative nuclear mRNA splicing, via spliceosome