Human wingless-type MMTV integration site family, member 7B ELISA Kit | WNT7B elisa kit

Human Protein Wnt-7b, WNT7B ELISA Kit

Cat. Num. AAA934421

• Reactivity: Human
• Synonyms: wingless-type MMTV integration site family; member 7B; Human Protein Wnt-7b; WNT7B ELISA Kit; Human Protein Wnt-7b (WNT7B) ELISA kit; WNT7B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human WNT7B. No significant cross-reactivity or interference between human WNT7B and analogues was observed.
• Sequence Length: 349

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml.
• Sensitivity: The minimum detectable dose of human WNT7B is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for WNT7B elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT7B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT7B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT7B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT7B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 17505193
• NCBI GeneID: 7477
• NCBI Accession #: NP_478679.1
• NCBI GenBank Nucleotide #: NM_058238.2
• UniProt Accession #: P56706; Q96Q12; B8A596
• Molecular Weight: 39,327 Da
• NCBI Official Full Name: protein Wnt-7b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 7B
• NCBI Official Symbol: WNT7B
• NCBI Protein Information: protein Wnt-7b
• UniProt Protein Name: Protein Wnt-7b
• Protein Family: Protein
• UniProt Gene Name: WNT7B
• UniProt Entry Name: WNT7B_HUMAN

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein. [provided by RefSeq, Oct 2008]

Uniprot Description

WNT7B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum lumen; Golgi lumen; plasma membrane; extracellular region

Molecular Function: frizzled binding

Biological Process: oxygen homeostasis; Wnt receptor signaling pathway; cell fate commitment; in utero embryonic development; response to glucocorticoid stimulus; positive regulation of JNK cascade; Wnt receptor signaling pathway through beta-catenin; forebrain regionalization; anatomical structure regression; activation of JNK activity; odontogenesis of dentine-containing teeth; neuron differentiation; positive regulation of osteoblast differentiation; cellular metabolic process; central nervous system vasculogenesis; fibroblast proliferation; homeostatic process; negative regulation of smoothened signaling pathway; positive regulation of cell proliferation; regulation of cell projection size; synapse organization and biogenesis; embryonic organ development; smooth muscle cell differentiation; establishment and/or maintenance of polarity of embryonic epithelium; neurite morphogenesis; lung development