Human Protein Wnt-2 ELISA Kit | WNT2 elisa kit

Human Protein Wnt-2 ELISA Kit

Cat. Num. AAA762001

• Gene Names: Wnt2; Irp; Mirp; Wnt-2; Wnt2a; Int1l1; 2610510E18Rik
• Reactivity: Human
• Synonyms: Protein Wnt-2; Human Protein Wnt-2 ELISA Kit; WNT2/NT1L1/IRP/Int-1-like protein 1/Int-1-related protein/secreted growth factor/Int-1-like protein 1/Int-1-related protein/IRPprotein Wnt-2/wingless-type MMTV integration site family member 2; WNT2 elisa kit

• Reactivity: Human
• Sequence Length: 360

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for WNT2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WNT2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WNT2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WNT2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 242397432
• NCBI GeneID: 22413
• NCBI Accession #: NP_076142.3
• NCBI GenBank Nucleotide #: NM_023653.5
• Molecular Weight: 40,482 Da
• NCBI Official Full Name: protein Wnt-2
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 2
• NCBI Official Symbol: Wnt2
• NCBI Official Synonym Symbols: Irp; Mirp; Wnt-2; Wnt2a; Int1l1; 2610510E18Rik
• NCBI Protein Information: protein Wnt-2
• UniProt Protein Name: Protein Wnt-2
• Protein Family: Protein
• UniProt Gene Name: Wnt2
• UniProt Synonym Gene Names: Irp; Wnt-2; IRP
• UniProt Entry Name: WNT2_MOUSE

Uniprot Description

WNT2: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Cell adhesion; Extracellular matrix; Cell development/differentiation; Secreted

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; receptor binding

Biological Process: atrial cardiac muscle morphogenesis; cell fate commitment; cell proliferation in midbrain; cell-cell signaling; multicellular organismal development; neuron differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of neurogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin