Sheep WNT1 Inducible Signaling Pathway Protein 1 ELISA Kit | WISP1 elisa kit

Sheep WNT1 Inducible Signaling Pathway Protein 1 ELISA Kit

Cat. Num. AAA752771

• Gene Names: WISP1; CCN4; WISP1c; WISP1i; WISP1tc
• Reactivity: Sheep
• Synonyms: WNT1 Inducible Signaling Pathway Protein 1; Sheep WNT1 Inducible Signaling Pathway Protein 1 ELISA Kit; WISP1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This WISP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep WISP1. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This WISP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep WISP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WISP1 elisa kit

Principle of the Assay: WISP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-WISP1 antibody and an WISP1-HRP conjugate. The assay sample and buffer are incubated together with WISP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WISP1 concentration since WISP1 from samples and WISP1-HRP conjugate compete for the anti-WISP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by WISP1 from the sample, fewer sites are left to bind WISP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WISP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for WISP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4507921
• NCBI GeneID: 8840
• NCBI Accession #: NP_003873.1
• NCBI GenBank Nucleotide #: NM_003882.3
• Molecular Weight: 21,550 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1 isoform 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 1
• NCBI Official Symbol: WISP1
• NCBI Official Synonym Symbols: CCN4; WISP1c; WISP1i; WISP1tc
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1; CTC-458A3.8; CCN family member 4; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP1
• UniProt Synonym Gene Names: CCN4; WISP-1
• UniProt Entry Name: WISP1_HUMAN

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Oncoprotein

Chromosomal Location of Human Ortholog: 8q24.22

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding

Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion