Human WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit | WISP1 elisa kit

Human WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit

Cat. Num. AAA2022092

• Gene Names: WISP1; CCN4; WISP1c; WISP1i; WISP1tc
• Reactivity: Human
• Synonyms: WNT1 Inducible Signaling Pathway Protein 1 (WISP1); Human WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit; CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein; WISP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT1 Inducible Signaling Pathway Protein 1 (WISP1).; No significant cross-reactivity or interference between WNT1 Inducible Signaling Pathway Protein 1 (WISP1) and analogues was observed.
• Sequence Length: 195

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 13.3pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WISP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT1 Inducible Signaling Pathway Protein 1 (WISP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT1 Inducible Signaling Pathway Protein 1 (WISP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for WISP1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4507921
• NCBI GeneID: 8840
• NCBI Accession #: NP_003873.1
• NCBI GenBank Nucleotide #: NM_003882.3
• UniProt Accession #: O95388; Q5JBS6; Q5JBS7; Q5JBS8; Q9HCS3; A8KAG6; E7EMM5
• Molecular Weight: 21,550 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1 isoform 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 1
• NCBI Official Symbol: WISP1
• NCBI Official Synonym Symbols: CCN4; WISP1c; WISP1i; WISP1tc
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WISP1
• UniProt Synonym Gene Names: CCN4; WISP-1
• UniProt Entry Name: WISP1_HUMAN

NCBI Description

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

WISP1: Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. Belongs to the CCN family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q24.22

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding

Biological Process: Wnt receptor signaling pathway; cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion