Mouse WD repeat-containing protein 61 ELISA Kit | WDR61 elisa kit

Mouse WD repeat-containing protein 61 (WDR61) ELISA Kit

Cat. Num. AAA282243

• Gene Names: Wdr61; REC14; 2700038L12Rik; 2810418I05Rik
• Reactivity: Mouse
• Synonyms: WD repeat-containing protein 61; Mouse WD repeat-containing protein 61 (WDR61) ELISA Kit; WDR61 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse WDR61. No significant cross-reactivity or interference between Mouse WDR61 and analogues was observed.
• Sequence Length: 305

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for WDR61 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WDR61 in samples. An antibody specific for WDR61 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR61 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDR61 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDR61 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 70778817
• NCBI GeneID: 66317
• NCBI Accession #: NP_001020546.1
• NCBI GenBank Nucleotide #: NM_001025375.2
• UniProt Accession #: Q9ERF3; Q3U562; Q9CZP1
• Molecular Weight: 33,773 Da
• NCBI Official Full Name: WD repeat-containing protein 61 isoform a
• NCBI Official Synonym Full Names: WD repeat domain 61
• NCBI Official Symbol: Wdr61
• NCBI Official Synonym Symbols: REC14; 2700038L12Rik; 2810418I05Rik
• NCBI Protein Information: WD repeat-containing protein 61
• UniProt Protein Name: WD repeat-containing protein 61
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: Wdr61

Uniprot Description

WDR61: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.

Chromosomal Location of Human Ortholog: 9|9 A5.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Biological Process: negative regulation of myeloid cell differentiation; positive regulation of histone H3-K4 methylation; positive regulation of transcription elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent; Wnt signaling pathway