Mouse WD repeat and FYVE domain-containing protein 3 ELISA Kit | WDFY3 elisa kit

Mouse WD repeat and FYVE domain-containing protein 3 (WDFY3) ELISA Kit

Cat. Num. AAA282880

• Gene Names: Wdfy3; ALFY; BWF1; Bchs; Ggtb3; ZFYVE25; AW319683; D5Ertd66e; mKIAA0993; B930017C24; 2610509D04Rik
• Reactivity: Mouse
• Synonyms: WD repeat and FYVE domain-containing protein 3; Mouse WD repeat and FYVE domain-containing protein 3 (WDFY3) ELISA Kit; WDFY3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse WDFY3. No significant cross-reactivity or interference between Mouse WDFY3 and analogues was observed.
• Sequence Length: 3526

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for WDFY3 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WDFY3 in samples. An antibody specific for WDFY3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDFY3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WDFY3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WDFY3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 39930599
• NCBI GeneID: 72145
• NCBI Accession #: NP_766470.2
• NCBI GenBank Nucleotide #: NM_172882.3
• UniProt Accession #: Q6VNB8; Q8C8H7
• Molecular Weight: 102,219 Da
• NCBI Official Full Name: WD repeat and FYVE domain-containing protein 3
• NCBI Official Synonym Full Names: WD repeat and FYVE domain containing 3
• NCBI Official Symbol: Wdfy3
• NCBI Official Synonym Symbols: ALFY; BWF1; Bchs; Ggtb3; ZFYVE25; AW319683; D5Ertd66e; mKIAA0993; B930017C24; 2610509D04Rik
• NCBI Protein Information: WD repeat and FYVE domain-containing protein 3
• UniProt Protein Name: WD repeat and FYVE domain-containing protein 3
• Protein Family: WD repeat and FYVE domain-containing protein
• UniProt Gene Name: Wdfy3
• UniProt Synonym Gene Names: BWF1

Uniprot Description

WDFY3: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Membrane protein, peripheral; Nuclear envelope; Transferase

Chromosomal Location of Human Ortholog: 5 E4-E5|5 48.95 cM

Cellular Component: autophagic vacuole; cytoplasm; cytosol; extrinsic component of membrane; inclusion body; membrane; nuclear envelope; nucleolus; nucleus; plasma membrane; PML body

Molecular Function: beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; metal ion binding; phosphatidylinositol binding

Biological Process: autophagy