Hepatocellular Carcinoma Related Protein 1 Recombinant Protein | HCRP1 recombinant protein

Recombinant Hepatocellular Carcinoma Related Protein 1 (HCRP1)

Cat. Num. AAA2033183

• Gene Names: VPS37A; HCRP1; PQBP2; SPG53
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hepatocellular Carcinoma Related Protein 1; Recombinant Hepatocellular Carcinoma Related Protein 1 (HCRP1); PQBP2; VPS37A; Vacuolar Protein Sorting 37 Homolog A; Polyglutamine Binding Protein 2; ESCRT-I complex subunit VPS37A; HCRP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MSWLFPLTKS ASSSAAGSPG GLTSLQQQKQ RLIESLRNSH SSIAEIQKDVEYRLPFTINN LTININILLP PQFPQEKPVI SVYPPIRHHL MDKQGVYVTSPLVNNFTMHS DLGKIIQSLL DEFWKNPPVL APTSTAFPYL YSNPSGMSPYASQGFPFLPP YPPQEANRSI TSLSVADTVS SSTTSHTTAK PAAPSFGVLSNLPLPIPTVD ASIPTSQNGF GYKMPDVPDA FPELSELSVS QLTDMNEQEEVLLEQFLTLP QLKQIITDKD DLVKSIEELA RKNLLLEPSL EAKRQTVLDKYELLTQMKST FEKKMQRQHE LSESCSASAL QARLKVAAHE AEEESDNIAEDFLEGKMEID DFLSSFMEKR TICHCRRAKE EKLQQAIAMH SQFHAPL
• Sequence Length: 397

• Applicable Applications for HCRP1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 48.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Leu397
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20384770
• NCBI GeneID: 137492
• Molecular Weight: 20,891 Da
• NCBI Official Full Name: hepatocellular carcinoma related protein 1
• NCBI Official Synonym Full Names: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• NCBI Official Symbol: VPS37A
• NCBI Official Synonym Symbols: HCRP1; PQBP2; SPG53
• NCBI Protein Information: vacuolar protein sorting-associated protein 37A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 37A
• UniProt Gene Name: VPS37A
• UniProt Synonym Gene Names: HCRP1; hVps37A
• UniProt Entry Name: VP37A_HUMAN

NCBI Description

This gene belongs to the VPS37 family, and encodes a component of the ESCRT-I (endosomal sorting complex required for transport I) protein complex, required for the sorting of ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies. Expression of this gene is downregulated in hepatocellular carcinoma, and mutations in this gene are associated with autosomal recessive spastic paraplegia-53. A related pseudogene has been identified on chromosome 5. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

VPS37A: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. Defects in VPS37A are a cause of autosomal recessive complex hereditary spastic paraparesis, a complicated form of hereditary spastic paraplegia (HSP). HSP is a heterogeneous group of neurodegenerative disorders characterized by progressive lower limb spasticity, retrograde degeneration of the crossed cortico- spinal tracts and thinning of the posterior columns in the spinal cord. Complicated forms are characterized by the addition of such neurological features as spastic quadriparesis, seizures, dementia, amyotrophy, extrapyramidal disturbance, cerebral or cerebellar atrophy, optic atrophy, and peripheral neuropathy, as well as by extra neurological manifestations such as dysmorphism, albinism, retinitis pigmentosa, deafness, dementia, amyotrophy and ichthyosis. Individuals with VPS37A mutations present with pronounced early onset spastic paraparesis of upper and lower limbs, mild intellectual disability, kyphosis, pectus carinatum and hypertrichosis. Belongs to the VPS37 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: centrosome; cytoplasm; endosome membrane; intracellular membrane-bound organelle; late endosome membrane; nucleoplasm

Molecular Function: protein binding

Biological Process: autophagy; endosome transport; protein transport; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral infectious cycle; viral protein processing; viral reproduction; virus assembly

Disease: Spastic Paraplegia 53, Autosomal Recessive