Typical Testing Data/Standard Curve (for reference only)
Mouse Vasoactive intestinal peptide ELISA Kit | VIP elisa kit
Mouse Vasoactive intestinal peptide ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: VIP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-VIP antibody and an VIP-HRP conjugate. The assay sample and buffer are incubated together with VIP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VIP concentration since VIP from samples and VIP-HRP conjugate compete for the anti-VIP antibody binding site. Since the number of sites is limited, as more sites are occupied by VIP from the sample, fewer sites are left to bind VIP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VIP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
VIP: VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6q25
Cellular Component: cell soma; extracellular region
Molecular Function: neuropeptide hormone activity; protein binding; hormone activity
Biological Process: negative regulation of smooth muscle cell proliferation; mRNA stabilization; negative regulation of potassium ion transport; regulation of signal transduction; positive regulation of vasodilation; body fluid secretion; G-protein coupled receptor protein signaling pathway; regulation of protein localization; learning and/or memory; positive regulation of cell proliferation; positive regulation of protein catabolic process; positive regulation of endothelial cell proliferation; regulation of sensory perception of pain; negative regulation of apoptosis
Research Articles on VIP
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Product Notes
The Mouse VIP vip (Catalog #AAA720553) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720553 ELISA Kit recognizes Mouse VIP. It is sometimes possible for the material contained within the vial of "Vasoactive intestinal peptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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