Silver Strain
(Hepatis A AntigenCat# MBS318674)
Hepatitis A Virus Antigens Antigen | HAV antigen
Hepatitis A Virus Antigen
Applications
Western Blot, Cytotoxicity Assay, ELISA
Synonyms
Hepatitis A Virus Antigens; Hepatitis A Virus Antigen; Hepatitis A (HAV) Virus Antigens; Hepatitis A Virus (HAV) Antigen; Concentrate; >60% Viral Protein; HAV antigen
Host
Grown in FRhK-4 Cells
Form/Format
Purified Liquid; NTE Buffer: 0.01M Tris, 0.15M Sodium Chloride, 1.0mM EDTA, pH 8.0-8.4, Formalin: < 1:2000 by volume
Applicable Applications for HAV antigen
Western Blot Assay, Silver Stain Assay, BCA Assay, ELISA Assay, Plaque Assay
Application Notes
Identity -
Western Blot Assay
Acceptable Result: Must demonstrate a major band in the region of 33kD and at least 1 band in the 24-27kD region. The 33kD band must co-migrate with that in the standard lane.
Testing Results: Pass
Silver Stain Assay
Acceptable Result: Must demonstrate a major band in the region of 33kD and at least 1 band in the 24-27kD region. The 33kD band must co-migrate with that in the standard lane.
Testing Results: Pass
EIA Titer -
ELISA Assay
Acceptable Range > 331
Testing Results: 346
Reference Antigen Lot Number: Q05211546
Reference Antigen Titer: 300
% Difference to Reference Antigen: 10%
Western Blot Assay
Acceptable Result: Must demonstrate a major band in the region of 33kD and at least 1 band in the 24-27kD region. The 33kD band must co-migrate with that in the standard lane.
Testing Results: Pass
Silver Stain Assay
Acceptable Result: Must demonstrate a major band in the region of 33kD and at least 1 band in the 24-27kD region. The 33kD band must co-migrate with that in the standard lane.
Testing Results: Pass
EIA Titer -
ELISA Assay
Acceptable Range > 331
Testing Results: 346
Reference Antigen Lot Number: Q05211546
Reference Antigen Titer: 300
% Difference to Reference Antigen: 10%
Viral Strain
pHM175
Preservative
0.085% Sodium Azide
Protein Concentration
BCA Assay:
Acceptable Range: > 25 ug/mL
Testing Results: 29 ug/mL
Acceptable Range: > 25 ug/mL
Testing Results: 29 ug/mL
Infectivity
Plaque Assay
Acceptable Results < 1 PFU/mL
Testing Results: 0 PFU/mL
Acceptable Results < 1 PFU/mL
Testing Results: 0 PFU/mL
Bioburden
Acceptable Range: 0 CFU/mL
Testing Results: 0 CFU/mL
Each laboratory should determine an optimum working titer for use in its particular application.
Testing Results: 0 CFU/mL
Each laboratory should determine an optimum working titer for use in its particular application.
Safety Note
Refer to the appropriate Safety Data Sheet (SDS) for additional information.
Preparation and Storage
Ships on dry ice. Store at < -65°C. Avoid multiple freeze/thaw cycles.
Silver Strain
(Hepatis A AntigenCat# MBS318674)
Silver Strain
(Hepatis A AntigenCat# MBS318674)
Western Blot (WB)
(Hepatis A AntigenCat# MBS318674)
Western Blot (WB)
(Hepatis A AntigenCat# MBS318674)
Related Product Information for HAV antigen
HAV Grade II Concentrate
Product Categories/Family for HAV antigen
Similar Products
Product Notes
The HAV (Catalog #AAA318674) is an Antigen produced from Grown in FRhK-4 Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hepatitis A Virus Antigens can be used in a range of immunoassay formats including, but not limited to, Western Blot Assay, Silver Stain Assay, BCA Assay, ELISA Assay, Plaque Assay. Identity - Western Blot Assay Acceptable Result: Must demonstrate a major band in the region of 33kD and at least 1 band in the 24-27kD region. The 33kD band must co-migrate with that in the standard lane. Testing Results: Pass Silver Stain Assay Acceptable Result: Must demonstrate a major band in the region of 33kD and at least 1 band in the 24-27kD region. The 33kD band must co-migrate with that in the standard lane. Testing Results: Pass EIA Titer - ELISA Assay Acceptable Range > 331 Testing Results: 346 Reference Antigen Lot Number: Q05211546 Reference Antigen Titer: 300 % Difference to Reference Antigen: 10%. Researchers should empirically determine the suitability of the HAV for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hepatitis A Virus Antigens, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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