Baculovirus Expression Vector pAc-k-Fc Vector
Baculovirus Expression Vector pAc-k-Fc
Purity
PurifiedPurified using a Plasmid Purification Kit.
Synonyms
Baculovirus Expression Vector pAc-k-Fc; Baculovirus Expression Vector pAc-k-Fc vector
Purity/Purification
Purified
Purified using a Plasmid Purification Kit.
Purified using a Plasmid Purification Kit.
Form/Format
Supplied as a liquid in TE buffer.
Application Notes
Cloning of heavy and light chain variable domain genes of scFv antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries.
Preparation and Storage
For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Baculovirus Expression Vector pAc-k-Fc vector
Baculovirus cassette vector pAc-k-Fc for the expression of human, humanized or chimeric IgG(kappa) in insect cells and secretion of assembled antibodies into the supernatant. The vector derived from pAcUW51 is suitable for cloning of heavy and light chain Fab antibody gene fragments.
Within the past decade, recombinant antibody technologies have been widely used to produce various single-chain Fv or Fab antibody fragments of different specificity. The randomized combination of cloned variable heavy and light chain immunoglobulin gene fragments further allowed the construction of human antibody libraries, which enable today the isolation of specific scFv or Fab antibody fragments against particular antigens e.g. by phage display. For many applications, however, it is required to reassemble the variable regions of the selected antibody with immunoglobulin constant regions to generate complete antibody molecules.
The baculovirus expression system has already been established as a reliable system for the production of immunoglobulins (Nesbit et al., 1992; Liang et al., 1997, 2001). Even the development of cassette vectors for the production of human antibodies have been reported previously (Poul et al., 1995a,b). However, these systems are based on a combination of two different vectors which separately served for the expression of light and heavy chains. The required careful and time consuming adjustment of the two respective recombinant baculovirus titers now becomes obsolete. Now available are single baculovirus cassette vectors with authentic IgG heavy and light chain signal sequences for the rapid production of complete chimeric, humanized and human IgG antibodies in recombinant baculovirus infected insect cells (Liang et al., 2001). The vectors were specifically designed for cloning of heavy and light chain Fv or Fab gene fragments isolated from hybridomas, individual B cell clones as well as antibody libraries.
Within the past decade, recombinant antibody technologies have been widely used to produce various single-chain Fv or Fab antibody fragments of different specificity. The randomized combination of cloned variable heavy and light chain immunoglobulin gene fragments further allowed the construction of human antibody libraries, which enable today the isolation of specific scFv or Fab antibody fragments against particular antigens e.g. by phage display. For many applications, however, it is required to reassemble the variable regions of the selected antibody with immunoglobulin constant regions to generate complete antibody molecules.
The baculovirus expression system has already been established as a reliable system for the production of immunoglobulins (Nesbit et al., 1992; Liang et al., 1997, 2001). Even the development of cassette vectors for the production of human antibodies have been reported previously (Poul et al., 1995a,b). However, these systems are based on a combination of two different vectors which separately served for the expression of light and heavy chains. The required careful and time consuming adjustment of the two respective recombinant baculovirus titers now becomes obsolete. Now available are single baculovirus cassette vectors with authentic IgG heavy and light chain signal sequences for the rapid production of complete chimeric, humanized and human IgG antibodies in recombinant baculovirus infected insect cells (Liang et al., 2001). The vectors were specifically designed for cloning of heavy and light chain Fv or Fab gene fragments isolated from hybridomas, individual B cell clones as well as antibody libraries.
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Product Notes
The Baculovirus Expression Vector pAc-k-Fc (Catalog #AAA641024) is a Vector and is intended for research purposes only. The product is available for immediate purchase. Cloning of heavy and light chain variable domain genes of scFv antibody fragments selected from hybridomas, single B-lymphocytes or phage display libraries. Researchers should empirically determine the suitability of the Baculovirus Expression Vector pAc-k-Fc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Baculovirus Expression Vector pAc-k-Fc, Vector" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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