VCAM1 recombinant protein

Recombinant Human VCAM1 Protein

Cat. Num. AAA9141839

• Gene Names: VCAM1; CD106; INCAM-100
• Purity: >95% by SDS-PAGE.
• Synonyms: VCAM1; Recombinant Human VCAM1 Protein; CD106; INCAM-100; VCAM-1; VCAM1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% by SDS-PAGE.
• Form/Format: Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
• Sequence: FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE
• Sequence Length: 739

• Species: Human
• Endotoxin: Please contact us for more information.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
• Tag: 6xHis tag at the C-terminus
• Preparation and Storage: Store the lyophilized protein at -20 degree C to -80 degree C for long term.; After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.

SDS-Page

(Recombinant Human VCAM1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-110 kDa.)

Related Product Information for VCAM1 recombinant protein

Description: Recombinant Human VCAM1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Phe25-Glu698) of human VCAM1 (Accession #NP_001069.1) fused with a 6xHis tag at the C-terminus.

Background: This protein also known as CD16, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and alpha4beta7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

Product Categories/Family for VCAM1 recombinant protein

CD Antigen

NCBI and Uniprot Product Information

• NCBI GI #: 137560
• NCBI GeneID: 7412
• UniProt Accession #: P19320
• NCBI Official Full Name: Vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• Protein Family: Vascular cell adhesion protein
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: L1CAM; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_HUMAN

NCBI Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; cell surface; microvillus; endoplasmic reticulum; early endosome; integral to membrane; apical part of cell; plasma membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: amine oxidase activity; integrin binding; cell adhesion molecule binding

Biological Process: response to nicotine; chronic inflammatory response; extracellular matrix organization and biogenesis; regulation of immune response; viral reproduction; cell-matrix adhesion; cytokine and chemokine mediated signaling pathway; heart development; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; amine metabolic process; response to ionizing radiation; cell adhesion; leukocyte tethering or rolling; response to nutrient; acute inflammatory response; aging