Mouse anti-Human PROK1 Monoclonal Antibody | anti-PROK1 antibody

PROK1 (Prokineticin-1, Endocrine-gland-derived Vascular Endothelial Growth Factor, EG-VEGF, Mambakine, UNQ600/PRO1186)

Cat. Num. AAA647892

• Gene Names: PROK1; PK1; PRK1; EGVEGF
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PROK1; Monoclonal Antibody; PROK1 (Prokineticin-1; Endocrine-gland-derived Vascular Endothelial Growth Factor; EG-VEGF; Mambakine; UNQ600/PRO1186); Anti -PROK1 (Prokineticin-1; anti-PROK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3C3
• Specificity: Recognizes human PROK1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MRGATRVSIMLLLVTVSDCAVITGACERDVQCGAGTCCAISLWLRGLRMCTPLGREGEECHPGSHKIPFFRKRKHHTCPCLPNLLCSRFPDGRYRCSMDLKNINF

• Applicable Applications for anti-PROK1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Immunogen: Full length recombinant corresponding to aa1-106 from human PROK1 (AAH25399) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged PROK1 is 0.3ng/ml as a capture antibody.)

Related Product Information for anti-PROK1 antibody

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.

Product Categories/Family for anti-PROK1 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 14165282
• NCBI GeneID: 84432
• NCBI Accession #: NP_115790.1
• NCBI GenBank Nucleotide #: NM_032414.2
• UniProt Accession #: P58294; Q5VWD4; Q8TC69
• Molecular Weight: 11,715 Da
• NCBI Official Full Name: prokineticin-1
• NCBI Official Synonym Full Names: prokineticin 1
• NCBI Official Symbol: PROK1
• NCBI Official Synonym Symbols: PK1; PRK1; EGVEGF
• NCBI Protein Information: prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor
• UniProt Protein Name: Prokineticin-1
• Protein Family: Prokineticin
• UniProt Gene Name: PROK1
• UniProt Synonym Gene Names: EG-VEGF
• UniProt Entry Name: PROK1_HUMAN

NCBI Description

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

Uniprot Description

Function: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Ref.1 Ref.2 Ref.9 Ref.10 Ref.11

Subcellular location: Secreted.

Tissue specificity: Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta. Ref.2 Ref.9 Ref.11

Developmental stage: In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.

Sequence similarities: Belongs to the AVIT (prokineticin) family.