Rabbit anti-Human MAP Kinase Kinase 7 Polyclonal Antibody | anti-MKK7 antibody

MAP Kinase Kinase 7, Phosphorylated (Thr275/Ser277) (Dual Specificity Mitogen-activated Protein Kinase Kinase 7, MAP2K7, MAPKK 7, MAPKK7, MKK7, PRKMK7, c-Jun N-terminal Kinase Kinase 2, JNK Kinase 2, JNKK 2, JNKK2, JNK-activating Kinase 2, MAPK/ERK Kinase

Cat. Num. AAA6486835

• Gene Names: MAP2K7; MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MAP Kinase Kinase 7; Polyclonal Antibody; Phosphorylated (Thr275/Ser277) (Dual Specificity Mitogen-activated Protein Kinase Kinase 7; MAP2K7; MAPKK 7; MAPKK7; MKK7; PRKMK7; c-Jun N-terminal Kinase Kinase 2; JNK Kinase 2; JNKK 2; JNKK2; JNK-activating Kinase 2; MAPK/ERK Kinase; anti-MKK7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MKK7/SKK4 when phosphorylated at Thr275 and Ser277, Mr 47kD. Species Crossreactivity: Human. Crossreactivity with mouse expected due to sequence homology.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MKK7 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000 detects recombinant MKK7 1 that had been phosphorylated by MEKK1. Recombinant MKK7 1 was left untreated or phosphorylated by MEKK1 and resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4 (KLH).
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: Recombinant MKK7b1 (M2363-95E) was left untreated (Lane 1) or phosphorylated by MEKK1 (M2865) (Lane 2) and probed with MBS629456 anti-phospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution). )

Product Categories/Family for anti-MKK7 antibody

Antibodies; Protein Kinases

References

1. Yoshida, K., et al., Mol. Cell. Biol. 20: 5370-5380, 2000. 2. Foltz, I., et al., J. Biol. Chem. 273: 9344-9351, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 21735544
• NCBI GeneID: 5609
• NCBI Accession #: NP_660186.1
• NCBI GenBank Nucleotide #: NM_145185.2
• UniProt Accession #: O14733; O14648; O14816; O60452; O60453; Q1PG43; Q8IY10; B2R9S5; D6W659
• Molecular Weight: 47,485 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 7
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: MAP2K7
• NCBI Official Synonym Symbols: MKK7; Jnkk2; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7; MEK 7; JNKK 2; MAPKK 7; SAPK kinase 4; MAPK/ERK kinase 7; MAP kinase kinase 7; JNK-activating kinase 2; c-Jun N-terminal kinase kinase 2; stress-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Protein Family: Mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K7
• UniProt Synonym Gene Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2
• UniProt Entry Name: MP2K7_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK7: a dual-specificity protein kinase of the STE7 family. Activates JNK1 and -2 by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by proinflammatory cytokines and environmental stress. Three alternatively spliced isoforms have been reported.

Protein type: Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.2; Kinase, protein; STE group; STE7 family

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: cytoplasm; intracellular; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein C-terminus binding; protein serine/threonine kinase activity; protein binding; enzyme binding; protein-tyrosine kinase activity; magnesium ion binding; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; protein kinase binding; protein phosphatase binding; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; response to osmotic stress; signal transduction; activation of JNK activity; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to heat; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; response to UV