Human CD106 ELISA Kit | VCAM1 elisa kit

Human CD106 ELISA Kit

Cat. Num. AAA8244720

• Gene Names: VCAM1; CD106; INCAM-100
• Reactivity: Human
• Applications: ELISA
• Synonyms: CD106; Human CD106 ELISA Kit; L1CAM; Vascular cell adhesion protein 1; V-CAM 1; VCAM-1; INCAM-100; VCAM1 elisa kit

• Reactivity: Human
• Specificity: The Human CD106 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD106 proteins within the range of 31.3 pg/ml - 2000 pg/ml.
• Sequence Length: 739

• Applicable Applications for VCAM1 elisa kit: Sandwich ELISA (EIA)
• Samples: Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates.
• Sensitivity: 7 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve

Related Product Information for VCAM1 elisa kit

Principle of the Assay||The Human CD106 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD106 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human CD106 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD106 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD106 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD106 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta 1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.

NCBI and Uniprot Product Information

• NCBI GI #: 137560
• NCBI GeneID: 7412
• UniProt Accession #: P19320; Q6NUP8; A8K6R7; B4DKS4; E9PDD1
• Molecular Weight: 74,346 Da
• NCBI Official Full Name: Vascular cell adhesion protein 1
• NCBI Official Synonym Full Names: vascular cell adhesion molecule 1
• NCBI Official Symbol: VCAM1
• NCBI Official Synonym Symbols: CD106; INCAM-100
• NCBI Protein Information: vascular cell adhesion protein 1
• UniProt Protein Name: Vascular cell adhesion protein 1
• Protein Family: Vascular cell adhesion protein
• UniProt Gene Name: VCAM1
• UniProt Synonym Gene Names: L1CAM; V-CAM 1; VCAM-1
• UniProt Entry Name: VCAM1_HUMAN

NCBI Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; microvillus; plasma membrane; podosome

Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding

Biological Process: amine metabolic process; B cell differentiation; cell adhesion; cell-matrix adhesion; extracellular matrix organization and biogenesis; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; regulation of immune response