Typical Testing Data/Standard Curve (for reference only)
Human Vascuolar Cell Adhesion Molecule 1 ELISA Kit | VCAM-1/CD106 elisa kit
Human VCAM-1/CD106 (Vascuolar Cell Adhesion Molecule 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VCAM-1/CD106. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for VCAM-1/CD106and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VCAM-1/CD106, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of VCAM-1/CD106.You can calculate the concentration of VCAM-1/CD106in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 1p32-p31
Cellular Component: apical part of cell; cell surface; early endosome; endoplasmic reticulum; external side of plasma membrane; extracellular space; filopodium; Golgi apparatus; integral to membrane; microvillus; plasma membrane; podosome; sarcolemma
Molecular Function: amine oxidase activity; cell adhesion molecule binding; integrin binding
Biological Process: acute inflammatory response; aging; amine metabolic process; B cell differentiation; cell adhesion; cell-matrix adhesion; chronic inflammatory response; cytokine and chemokine mediated signaling pathway; extracellular matrix organization and biogenesis; heart development; heterophilic cell adhesion; leukocyte adhesion; leukocyte tethering or rolling; membrane to membrane docking; positive regulation of T cell proliferation; regulation of immune response; response to ethanol; response to hypoxia; response to ionizing radiation; response to lipopolysaccharide; response to nicotine; response to nutrient; response to zinc ion; viral reproduction
Research Articles on VCAM-1/CD106
Similar Products
Product Notes
The Human VCAM-1/CD106 vcam1 (Catalog #AAA2533402) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533402 ELISA Kit recognizes Human VCAM-1/CD106. It is sometimes possible for the material contained within the vial of "Vascuolar Cell Adhesion Molecule 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
