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Varicella Zoster Virus ORF9 Recombinant Protein | VZV ORF9 recombinant protein

Varicella Zoster Virus ORF9 Recombinant

Purity
>95%, as determined by SDS-PAGE.
Synonyms
Varicella Zoster Virus ORF9; Varicella Zoster Virus ORF9 Recombinant; vzv; orf9; varicella zoster; VZV ORF9 recombinant protein
Ordering
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE.
Form/Format
Lyophilized from 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
Sequence Length
302
Application Notes
VZV ORF9 antigen is suitable for ELISA and Western blots, excellent antigen for detection of VZV with minimal specificity problems.
Host Note
DNA sequence encoding immunodominant fragment of Varicella Zoster virus ORF9 protein was expressed in Escherichia Coli.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Recombinant Varicella Zoster virus ORF9 encodes the immunodominant sequences of the VZV ORF9 protein. This molecule has a calculated mass of approximately 5kDa.

Recombinant VZV ORF9 contains an N terminal GST purification tag and migrates as an approximately 31 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.
Upon reconstitution, this protein can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Related Product Information for VZV ORF9 recombinant protein
The varicella-zoster virus (VZV) ORF9 protein is a member of the herpesvirus UL49 gene family but shares limited identity and similarity with the UL49 prototype, herpes simplex virus type 1 VP22. ORF9 mRNA is the most abundantly expressed message during VZV infection. The VZV ORF9 protein has not been well characterized, despite the fact that its transcript is the most abundant viral message expressed during lytic infection.
Product Categories/Family for VZV ORF9 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
32,816 Da
NCBI Official Full Name
Tegument protein VP22
UniProt Protein Name
Tegument protein VP22
UniProt Entry Name
VP22_VZVO

Uniprot Description

Function: Tegument protein that may play a role in the nucleocapsid secondary envelopment at the trans-Golgi network during virus morphogenesis

Potential.

Subunit structure: Interacts with gE (via C-terminus); this interaction is necessary for the recruitment of VP22 to the Golgi and its packaging into virions. Interacts with VP16

By similarity. Ref.3

Subcellular location: Virion tegument

By similarity. Host cytoplasm

By similarity. Host nucleus

By similarity. Host Golgi apparatus

By similarity. Note: One of the most abundant tegument protein. During virion morphogenesis, this protein probably accumulates at the trans-Golgi where secondary envelopment occurs

By similarity. Ref.3

Post-translational modification: Highly phosphorylated in the host cell. Packaging is selective for underphosphorylated forms

By similarity.

Sequence similarities: Belongs to the alphaherpesvirinae VP22 tegument protein family.

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Product Notes

The VZV ORF9 (Catalog #AAA553166) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. VZV ORF9 antigen is suitable for ELISA and Western blots, excellent antigen for detection of VZV with minimal specificity problems. . Researchers should empirically determine the suitability of the VZV ORF9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Varicella Zoster Virus ORF9, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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