uSP46 recombinant protein

uSP46, 1-366aa, Human, His tag, E Coli

Cat. Num. AAA205710

• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: uSP46; 1-366aa; Human; His tag; E Coli; ubiquitin carboxyl-terminal hydrolase 46 isoform 1; Deubiquitinating enzyme 46; FLJ12552; ubiquitin carboxyl-terminal hydrolase 46; ubiquitin thioesterase 46; ubiquitin-specific-processing protease 46; uSP46 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTVRNIA SICNMGTNAS ALEKDIGPEQ FPINEHYFGL VNFGNTCYCN SVLQALYFCR PFRENVLAYK AQQKKKENLL TCLADLFHSI ATQKKKVGVI PPKKFISRLR KENDLFDNYM QQDAHEFLNY LLNTIADILQ EEKKQEKQNG KLKNGNMNEP AENNKPELTW VHEIFQGTLT NETRCLNCET VSSKDEDFLD LSVDVEQNTS ITHCLRDFSN TETLCSEQKY YCETCCSKQE AQKRMRVKKL PMILALHLKR FKYMEQLHRY TKLSYRVVFP LELRLFNTSS DAVNLDRMYD LVAVVVHCGS GPNRGHYITI VKSHGFWLLF DDDIVEKIDA QAIEEFYGLT SDISKNSESG YILFYQSRE
• Sequence Length: 366

• Applicable Applications for uSP46 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uSP46 recombinant protein

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. uSP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. Recombinant human uSP46 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for uSP46 recombinant protein

Epigenomics (Transcription & Translation)

References

Fukuo,Y., et al. (2011) J Affect Disord 133 (1-2), 150-157; Zhang,W., et al. (2011) PLoS ONE 6 (10), E26297;

NCBI and Uniprot Product Information

• NCBI GI #: 31377709
• NCBI GeneID: 64854
• NCBI Accession #: NP_073743
• NCBI GenBank Nucleotide #: NM_022832.3
• UniProt Accession #: P62068; Q80V95; Q9H7U4; Q9H9T8; B7Z3Y7; B7Z675; B7Z7S3; G8ACC7
• Molecular Weight: 44.8kDa (389aa) confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 46 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 46
• NCBI Official Symbol: USP46
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 46
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 46
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP46

NCBI Description

Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP46 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Jun 2009]

Uniprot Description

Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67 (). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:19075014, PubMed:26388029). Not involved in deubiquitination of monoubiquitinated FANCD2 (PubMed:19075014).