uPP1 recombinant protein

uPP1, 1-310aa, Human, His tag, E Coli

Cat. Num. AAA203502

• Gene Names: UPP1; UP; UPP; UPASE; UDRPASE
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: uPP1; 1-310aa; Human; His tag; E Coli; uridine phosphorylase 1; uP; uPASE; uPP; urdPase 1; uPP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 200mM NaCl
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF GDVKFVCVGG SPSRMKAFIR CVGAELGLDC PGRDYPNICA GTDRYAMYKV GPVLSVSHGM GIPSISIMLH ELIKLLYYAR CSNVTIIRIG TSGGIGLEPG TVVITEQAVD TCFKAEFEQI VLGKRVIRKT DLNKKLVQEL LLCSAELSEF TTVVGNTMCT LDFYEGQGRL DGALCSYTEK DKQAYLEAAY AAGVRNIEME SSVFAAMCSA CGLQAAVVCV TLLNRLEGDQ ISSPRNVLSE YQQRPQRLVS YFIKKKLSKA
• Sequence Length: 36

• Applicable Applications for uPP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uPP1 recombinant protein

uridine phosphorylase 1, also known as uPP1 catalyses the reversible phosphorolysis of uridine to uracil. The reaction products are then utilized as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis. The expression levels and the enzymatic activity of uPP1 are higher in human solid tumors than in adjacent normal tissues. In addition, uPP1 controls the homeostatic regulation of uridine concentration in plasma and tissues and plays a role in the intracellular activation of 5-fluorouracil. Recombinant human uPP1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for uPP1 recombinant protein

Cancer

References

Kanzaki A., et al. (2002) Int J Cancer. 97 (5):631-5.; Russll RL., et al. (2001) J Biol Chem. 276 (16):13302-7.;

NCBI and Uniprot Product Information

• NCBI GI #: 31742508
• NCBI GeneID: 7378
• NCBI Accession #: NP_853628
• NCBI GenBank Nucleotide #: NM_181597.1
• Molecular Weight: 36 kDa (330aa), confirmed by MALDI-TOF
• NCBI Official Synonym Full Names: uridine phosphorylase 1
• NCBI Official Symbol: UPP1
• NCBI Official Synonym Symbols: UP; UPP; UPASE; UDRPASE
• NCBI Protein Information: uridine phosphorylase 1
• UniProt Protein Name: Uridine phosphorylase 1
• Protein Family: Uridine phosphorylase
• UniProt Gene Name: UPP1
• UniProt Synonym Gene Names: UP; UPase 1; UrdPase 1

NCBI Description

This gene encodes a uridine phosphorylase, an enzyme that catalyzes the reversible phosphorylation of uridine (or 2'- deoxyuridine) to uracil and ribose-1-phosphate (or deoxyribose-1-phosphate). The encoded enzyme functions in the degradation and salvage of pyrimidine ribonucleosides. [provided by RefSeq, Oct 2016]

Uniprot Description

Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate (PubMed:7488099). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.