Uridine Phosphorylase 1 Recombinant Protein | UPP1 recombinant protein

Recombinant Uridine Phosphorylase 1 (UPP1)

Cat. Num. AAA2033219

• Gene Names: Upp1; Up; Upp; UPase; UdRPase; AI325217
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 90%
• Synonyms: Uridine Phosphorylase 1; Recombinant Uridine Phosphorylase 1 (UPP1); UDRPASE; UP; UPASE; UPP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MAATGTEAKD LENHHNDCFI QLSNPNIAAM KEDVLYHFNL STSTHDFPAMFGDVKFVCVG GSSSRMNTFI KYVAAELGLD HPGKEYPNIC AGTDRYAMYKAGPVLSVSHG MGIPSIGIML HELIKMLYHA RCSNITIIRI GTSGGIGLEPGSVVITQQAV NECFKPEFEQ IVLGKRVIRN TNLDAQLVQE LVQCSSDLNEFPMVVGNTMC TLDFYEGQGR LDGALCSYTE KDKQSYLRAA HAAGVRNIEMESSVFATMCS ACGLKAAVVC VTLLDRL
• Sequence Length: 311

• Applicable Applications for UPP1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 33.9kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Leu277
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 226874860
• NCBI GeneID: 22271
• NCBI Accession #: NP_001152874.1
• NCBI GenBank Nucleotide #: NM_001159402.1
• Molecular Weight: 34,086 Da
• NCBI Official Full Name: uridine phosphorylase 1
• NCBI Official Synonym Full Names: uridine phosphorylase 1
• NCBI Official Symbol: Upp1
• NCBI Official Synonym Symbols: Up; Upp; UPase; UdRPase; AI325217
• NCBI Protein Information: uridine phosphorylase 1
• UniProt Protein Name: Uridine phosphorylase 1
• Protein Family: Uridine phosphorylase
• UniProt Gene Name: Upp1
• UniProt Synonym Gene Names: Up; Upp; UPase 1; UrdPase 1
• UniProt Entry Name: UPP1_MOUSE

Uniprot Description

UPP1: Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1- phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Belongs to the PNP/UDP phosphorylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - pyrimidine; Transferase; EC 2.4.2.3; Xenobiotic Metabolism - drug metabolism - other enzymes

Cellular Component: cytoplasm

Molecular Function: catalytic activity; transferase activity; transferase activity, transferring glycosyl groups; transferase activity, transferring pentosyl groups; uridine phosphorylase activity

Biological Process: nucleoside metabolic process; nucleotide catabolic process; pyrimidine nucleotide metabolic process; uridine catabolic process; uridine metabolic process