Mouse SUN domain-containing protein 2 ELISA Kit | UNC84B elisa kit

Mouse SUN domain-containing protein 2 (UNC84B) ELISA Kit

Cat. Num. AAA2604922

• Gene Names: SUN2; UNC84B
• Reactivity: Mouse
• Synonyms: SUN domain-containing protein 2; Mouse SUN domain-containing protein 2 (UNC84B) ELISA Kit; UNC84B elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 717

• Samples: Serum, plasma or cell culture supernatant and organizations in the natural and recombinant UNC84B concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UNC84B elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse UNC84B monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 31742498
• NCBI GeneID: 25777
• NCBI Accession #: NP_056189.1
• NCBI GenBank Nucleotide #: NM_015374.2
• Molecular Weight: 79,612 Da
• NCBI Official Full Name: SUN domain-containing protein 2 isoform b
• NCBI Official Synonym Full Names: Sad1 and UNC84 domain containing 2
• NCBI Official Symbol: SUN2
• NCBI Official Synonym Symbols: UNC84B
• NCBI Protein Information: SUN domain-containing protein 2
• UniProt Protein Name: SUN domain-containing protein 2
• Protein Family: SUN domain-containing protein
• UniProt Gene Name: SUN2
• UniProt Synonym Gene Names: FRIGG; KIAA0668; RAB5IP; UNC84B; Rab5IP
• UniProt Entry Name: SUN2_HUMAN

NCBI Description

SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010]

Uniprot Description

SUN2: a nuclear transmembrane protein. Forms part of a membrane-spanning fibrillar complex that interconnects attached meiotic telomeres with cytoplasmic structures. Together with SUN1 connects the nuclear envelope with the centrosome. Interacts with RAB5A. Reportedly expressed in the endosome where it may play a role in endosome fusion and endocytosis.

Protein type: Nuclear envelope; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: condensed nuclear chromosome; endosome membrane; integral to nuclear inner membrane; nuclear chromosome, telomeric region; nuclear envelope; nuclear membrane

Molecular Function: identical protein binding; lamin binding; microtubule binding; protein anchor; protein binding

Biological Process: centrosome localization; mitotic spindle organization and biogenesis; nuclear membrane organization and biogenesis; nuclear migration; nuclear migration along microfilament; positive regulation of cell migration