UDP Glucose Ceramide Glucosyltransferase Recombinant Protein | UGCG recombinant protein

Recombinant UDP Glucose Ceramide Glucosyltransferase (UGCG)

Cat. Num. AAA2031754

• Gene Names: UGCG; GCS; GLCT1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: UDP Glucose Ceramide Glucosyltransferase; Recombinant UDP Glucose Ceramide Glucosyltransferase (UGCG); UGCG recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 6.7 (varies by lot)
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-KK ATDKQPYSKL PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK LLGKYPNVDA RLFIGGKKVG INPKINNLMP GYEVAKYDLIWICDSGIRVI PDTLTDMVNQ MTEKVGLVHG L
• Sequence Length: 394

• Applicable Applications for UGCG recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 16.4kDa
• Accurate Molecular Mass (KD): 16kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Multi-species
• Fragment: Lys39~Leu171
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 24270822
• NCBI GeneID: 7357
• Molecular Weight: 44,854 Da
• NCBI Official Full Name: UDP-glucose ceramide glucosyltransferase
• NCBI Official Synonym Full Names: UDP-glucose ceramide glucosyltransferase
• NCBI Official Symbol: UGCG
• NCBI Official Synonym Symbols: GCS; GLCT1
• NCBI Protein Information: ceramide glucosyltransferase
• UniProt Protein Name: Ceramide glucosyltransferase
• Protein Family: Ceramide glucosyltransferase
• UniProt Gene Name: UGCG
• UniProt Synonym Gene Names: GCS
• UniProt Entry Name: CEGT_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the first glycosylation step in the biosynthesis of glycosphingolipids, which are membrane components containing lipid and sugar moieties. The product of this reaction is glucosylceramide, which is the core structure of many glycosphingolipids. [provided by RefSeq, Dec 2014]

Uniprot Description

UGCG: Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a flippase. Belongs to the glycosyltransferase 2 family.

Protein type: Transferase; Lipid Metabolism - sphingolipid; Membrane protein, multi-pass; Membrane protein, integral; EC 2.4.1.80

Chromosomal Location of Human Ortholog: 9q31

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: ceramide glucosyltransferase activity

Biological Process: epidermis development; glycosphingolipid biosynthetic process; sphingolipid metabolic process; glucosylceramide biosynthetic process; glycosphingolipid metabolic process