Horse Uncoupling Protein 1, Mitochondrial ELISA Kit | UCP1 elisa kit

Horse Uncoupling Protein 1, Mitochondrial ELISA Kit

Cat. Num. AAA66225

• Gene Names: PUMP1; ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1; ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1; ATPUMP1; ATUCP1; plant uncoupling mitochondrial protein 1; PUMP1; UCP; UCP1; UNCOUPLING PROTEIN 1
• Reactivity: Horse
• Synonyms: Uncoupling Protein 1; Mitochondrial; Horse Uncoupling Protein 1; Mitochondrial ELISA Kit; UCP1 elisa kit

• Reactivity: Horse
• Specificity: No significant cross-reactivity or interference between Horse UCP1 and analogues was observed.
• Sequence Length: 306

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 62.5 pg/ml - 2000 pg/ml.
• Sensitivity: 10 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated UCP1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse UCP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCP1 and UCP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for UCP1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated UCP1 concentrations in Horse serum, plasma and other body fluids. Using Purified Horse UCP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCP1 and UCP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 15232420
• NCBI GeneID: 824578
• NCBI Accession #: NP_190979.1
• NCBI GenBank Nucleotide #: NM_115271.4
• UniProt Accession #: O81845; O65623; Q56Z57
• Molecular Weight: 32,662 Da
• NCBI Official Full Name: uncoupling family protein PUMP2
• NCBI Official Symbol: PUMP1
• NCBI Official Synonym Symbols: ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1; ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1; ATPUMP1; ATUCP1; plant uncoupling mitochondrial protein 1; PUMP1; UCP; UCP1; UNCOUPLING PROTEIN 1
• NCBI Protein Information: uncoupling family protein PUMP2
• UniProt Protein Name: Mitochondrial uncoupling protein 1
• Protein Family: Mitochondrial brown fat uncoupling protein
• UniProt Gene Name: PUMP1
• UniProt Synonym Gene Names: UCP1; AtPUMP1
• UniProt Entry Name: PUMP1_ARATH

NCBI Description

Member of Uncoupling protein PUMP2 family. Encodes a mitochondrial uncoupling protein AtUCP1 involved in maintain the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. Disruption of UCP1 results in a photosynthetic phenotype. Specifically there is a restriction in photorespiration with a decrease in the rate of oxidation of photorespiratory glycine in the mitochondrion. This change leads to an associated reduced photosynthetic carbon assimilation rate.

Uniprot Description

Function: PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration. Ref.8 Ref.9 Ref.12 Ref.13 Ref.15 Ref.16

Subcellular location: Mitochondrion inner membrane; Multi-pass membrane protein

By similarity.

Tissue specificity: Widely expressed. Ref.1 Ref.12

Induction: By cold stress, high light and chloramphenicol. Ref.1 Ref.10 Ref.14

Disruption phenotype: Slight reduction of shoot mass. Ref.12

Miscellaneous: Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt (Ref.9).

Sequence similarities: Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]Contains 3 Solcar repeats.

Sequence caution: The sequence BAD95028.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.