UCHL1 recombinant protein

UCHL1 recombinant protein

Cat. Num. AAA515402

• Gene Names: UCHL1; PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• Applications: Western Blot
• Synonyms: UCHL1; UCHL1 recombinant protein; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 1141

• Applicable Applications for UCHL1 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant full length human UCHL1 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for UCHL1 recombinant protein

Recombinant full length human UCHL1 was expressed in E. coli cells using a N-terminal His tag.

Scientific Background: UCHL1 is a member of the peptidase C12 family and a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to generate the ubiquitin monomer. UCHL1 is expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in UCHL1 may be associated with Parkinson disease (1). The ligase and hydrolase activities of UCHL1 may play roles in proteasomal protein degradation, a process critical for neuronal health. Inhibition of UCHL1 in mouse hippocampal slices reduces normal synaptic function and long-term potentiation. PKA-CREB pathway mediates the effects of UCHL1 on Amyloid-beta-induced synaptic dysfunction and Uchl1 also reversed the inhibition of CREB phosphorylation induced by Amyloid-beta (2).

Product Categories/Family for UCHL1 recombinant protein

Ubiquitin Proteins; Signaling Proteins - Ubiquitin Proteins

References

1. Liu, Y. et al: The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Cell 111: 209-218, 2002.
2. Gong, B. et al: Ubiquitin hydrolase Uch-L1 rescues beta-amyloid-induced decreases in synaptic function and contextual memory. Cell 126: 775-788, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 239835751
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NM_004181
• UniProt Accession #: P09936; Q4W5K6; Q71UM0
• Molecular Weight: ~25 kDa
• NCBI Official Full Name: Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1), mRNA
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: PARK5; PGP95; PGP9.5; Uch-L1; PGP 9.5
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Uniprot Description

Function: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Ref.12 Ref.13 Ref.28

Catalytic activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). Ref.20

Subunit structure: Monomer. Homodimer. Interacts with SNCA

By similarity. Interacts with COPS5. Ref.14 Ref.19

Subcellular location: Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note: About 30% of total UCHL1 is associated with membranes in brain. Ref.16

Tissue specificity: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Ref.5 Ref.12

Post-translational modification: O-glycosylated

By similarity.

Involvement in disease: Parkinson disease 5 (PARK5) [MIM:613643]: A complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Clinical features include parkinsonian symptoms (resting tremor, rigidity, postural instability and bradykinesia), dementia, diffuse Lewy body pathology, autonomic dysfunction, hallucinations and paranoia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.8 Ref.13 Ref.21 Ref.27

Miscellaneous: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles. In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.

Sequence similarities: Belongs to the peptidase C12 family.

Caution: Ref.8 reports the association of mutation Ile93Met with Parkinson disease. However, according to Ref.27 this association is uncertain and UCHL1 is not a susceptibility gene for Parkinson disease.

Biophysicochemical propertiesKinetic parameters:KM=122 nM for Ub-AMC Ref.8 Ref.11 Ref.21KM=1.20 µM for ubiquitin ethyl esterVmax=0.47 µmol/min/mg enzyme toward Ub-AMCVmax=25 µmol/min/mg enzyme toward ubiquitin ethyl ester

Sequence caution: The sequence CAA28443.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.