Ubiquitin Conjugating Enzyme E2 L3 Recombinant Protein | Ube2l3 recombinant protein

Ubiquitin Conjugating Enzyme E2 L3, Recombinant, Human (Ubc7)

Cat. Num. AAA636061

• Gene Names: Ube2l3; UbcM4; Ubce7; C79827
• Purity: Highly Purified; 95% by RP-HPLC and SDS-PAGE
• Synonyms: Ubiquitin Conjugating Enzyme E2 L3; Recombinant; Human (Ubc7); Ube2l3 recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% by RP-HPLC and SDS-PAGE
• Form/Format: Supplied as a liquid in 50mM HEPES, pH 7.5, 150mM sodium chloride, 1mM DTT, 10% glycerol.
• Sequence: MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD

• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Ube2l3 recombinant protein

Human Ubquitin-conjugating enzyme 7(UbcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kB maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes(E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human UBE2L1-UBE2L4 gene could potentially encode different isoforms of the UbcH7. UBE2L3 gene, located at chromosome 22q11.2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of UbcH7.

Recombinant Human UbC7 produced in E.coli is a single, non-glycosylated polypeptide chain containing 154 amino acids & having a molecular mass of 17.9kD.

Product Categories/Family for Ube2l3 recombinant protein

Molecular Biology; MB-Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 6678481
• NCBI GeneID: 22195
• NCBI Accession #: NP_033482.1
• NCBI GenBank Nucleotide #: NM_009456.2
• UniProt Accession #: P68037; P51966; P70653; Q9HAV1
• Molecular Weight: 17.9kD
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 L3
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2L 3
• NCBI Official Symbol: Ube2l3
• NCBI Official Synonym Symbols: UbcM4; Ubce7; C79827
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 L3; ubiquitin-protein ligase L3; ubiquitin carrier protein L3; ubiquitin-conjugating enzyme 7
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 L3
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: Ube2l3
• UniProt Synonym Gene Names: Ubce7
• UniProt Entry Name: UB2L3_MOUSE

Uniprot Description

UBE2L3: Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PARK2 and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down- regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis. Interacts with PARK2; involved in ubiquitination and degradation of misfolded proteins. Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53. Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B. Interacts with ARIH1. Interacts with ARIH2 (via RING-type 1). Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1. Ubiquitous, with highest expression in testis. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase; Nuclear receptor co-regulator

Cellular Component: cytoplasm; nucleus

Molecular Function: ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity

Biological Process: cell proliferation; protein polyubiquitination; regulation of transcription, DNA-dependent; positive regulation of protein ubiquitination; positive regulation of ubiquitin-protein ligase activity; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination