Human SUMO conjugating enzyme UBC9 (UBE2I) ELISA Kit | UBE2I elisa kit

Human SUMO conjugating enzyme UBC9 (UBE2I) ELISA Kit

Cat. Num. AAA7253244

• Gene Names: UBE2I; P18; UBC9; C358B7.1
• Reactivity: Human
• Synonyms: SUMO conjugating enzyme UBC9 (UBE2I); Human SUMO conjugating enzyme UBC9 (UBE2I) ELISA Kit; UBE2I elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This UBE2I ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human UBE2I. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UBE2I elisa kit

Principle of the assay: UBE2I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UBE2I antibody and an UBE2I-HRP conjugate. The assay sample and buffer are incubated together with UBE2I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UBE2I concentration since UBE2I from samples and UBE2I-HRP conjugate compete for the anti-UBE2I antibody binding site. Since the number of sites is limited, as more sites are occupied by UBE2I from the sample, fewer sites are left to bind UBE2I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UBE2I concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 35494003
• NCBI GeneID: 7329
• NCBI Accession #: NP_919237.1
• NCBI GenBank Nucleotide #: NM_194261.2
• Molecular Weight: 18,007 Da
• NCBI Official Full Name: SUMO-conjugating enzyme UBC9
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2I
• NCBI Official Symbol: UBE2I
• NCBI Official Synonym Symbols: P18; UBC9; C358B7.1
• NCBI Protein Information: SUMO-conjugating enzyme UBC9; SUMO-protein ligase; SUMO-1-protein ligase; ubiquitin-protein ligase I; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase E2I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
• UniProt Protein Name: SUMO-conjugating enzyme UBC9
• UniProt Gene Name: UBE2I
• UniProt Synonym Gene Names: UBC9; UBCE9
• UniProt Entry Name: UBC9_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UBC9: Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Interacts with HIPK1, HIPK2, PPM1J, RASD2 and TCF3 Interacts with NR2C1; the interaction promotes its sumoylation. Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B. Interacts with DNMT1. Interacts with FOXL2. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Interacts with human herpesvirus 6 IE2. Interacts with human adenovirus early E1A protein; this interaction interferes with polysumoylation (Probable). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain. Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML- RARA oncoprotein. Interacts with IPO13. Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. Belongs to the ubiquitin-conjugating enzyme family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Nuclear receptor co-regulator; SUMO conjugating system; Ubiquitin ligase; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; PML body; dendrite; cytoplasm; fibrillar center; synapse; synaptonemal complex; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; ubiquitin protein ligase binding; bHLH transcription factor binding; transcription factor binding; HLH domain binding; ATP binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; mitosis; viral reproduction; positive regulation of steroid hormone receptor signaling pathway; protein modification process; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; post-translational protein modification; chromosome segregation; protein sumoylation; cellular protein metabolic process; cell division; positive regulation of transcription factor activity; regulation of receptor activity; negative regulation of transcription, DNA-dependent