Ubiquitin Conjugating Enzyme E2F Recombinant Protein | UBE2F recombinant protein
Recombinant Ubiquitin Conjugating Enzyme E2F (UBE2F)
Gene Names
UBE2F; NCE2
Applications
SDS-Page, Western Blot
Purity
>95%
Synonyms
Ubiquitin Conjugating Enzyme E2F; Recombinant Ubiquitin Conjugating Enzyme E2F (UBE2F); NCE2; NEDD8 Conjugating Enzyme; NEDD8-conjugating enzyme 2; NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; UBE2F recombinant protein
Host
E Coli
Purity/Purification
>95%
Form/Format
Freeze-dried powder
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence Positions
Met1~Arg185
Sequence Length
185
Applicable Applications for UBE2F recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His Tag
Subcellular Location
Secreted
Isoelectric Point
6.3
Reconstitution
Reconstitute in PBS or others.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Product Categories/Family for UBE2F recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 24.8kDa
Accurate Molecular Mass: 25kDa
Accurate Molecular Mass: 25kDa
NCBI Official Full Name
NEDD8-conjugating enzyme UBE2F
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 F (putative)
NCBI Official Symbol
UBE2F
NCBI Official Synonym Symbols
NCE2
NCBI Protein Information
NEDD8-conjugating enzyme UBE2F
UniProt Protein Name
NEDD8-conjugating enzyme UBE2F
Protein Family
UniProt Gene Name
UBE2F
UniProt Synonym Gene Names
NCE2
UniProt Entry Name
UBE2F_HUMAN
Uniprot Description
UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 2q37.3
Cellular Component: cytoplasm
Molecular Function: protein binding; ubiquitin protein ligase binding; ATP binding; NEDD8 ligase activity; ligase activity
Biological Process: protein neddylation; protein ubiquitination
Research Articles on UBE2F
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Product Notes
The UBE2F ube2f (Catalog #AAA2123269) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Met1~Arg185. AAA Biotech's Ubiquitin Conjugating Enzyme E2F can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the UBE2F ube2f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin Conjugating Enzyme E2F, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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