uBE2C recombinant protein

uBE2C, 1-179aa, Human, His tag, E Coli

Cat. Num. AAA204396

• Gene Names: UBE2C; UBCH10; dJ447F3.2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: uBE2C; 1-179aa; Human; His tag; E Coli; ubiquitin-conjugating enzyme E2 C; dJ447F3.2; uBCH10; uBE2C recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASQNRD PAATSVAAAR KGAEPSGGAA RGPVGKRLQQ ELMTLMMSGD KGISAFPESD NLFKWVGTIH GAAGTVYEDL RYKLSLEFPS GYPYNAPTVK FLTPCYHPNV DTQGNICLDI LKEKWSALYD VRTILLSIQS LLGEPNIDSP LNTHAAELWK NPTAFKKYLQ ETYSKQVTSQ EP
• Sequence Length: 179

• Applicable Applications for uBE2C recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for uBE2C recombinant protein

ubiquitin-conjugating enzyme E2 C, also known as uBE2C, is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Recombinant human uBE2C protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for uBE2C recombinant protein

Ubiquitination

References

Okamoto Y., et al. (2003) Cancer Res. 63:4167-4173; Wagner K.W., et al. (2004) Oncogene. 23:6621-6629

NCBI and Uniprot Product Information

• NCBI GI #: 5902146
• NCBI GeneID: 11065
• NCBI Accession #: NP_008950
• NCBI GenBank Nucleotide #: NM_007019.3
• UniProt Accession #: O00762; A6NP33; E1P5N7; G3XAB7
• Molecular Weight: 22.1 kDa (202aa), confirmed by MALDI-TOF
• NCBI Official Full Name: ubiquitin-conjugating enzyme E2 C isoform 1
• NCBI Official Synonym Full Names: ubiquitin conjugating enzyme E2 C
• NCBI Official Symbol: UBE2C
• NCBI Official Synonym Symbols: UBCH10; dJ447F3.2
• NCBI Protein Information: ubiquitin-conjugating enzyme E2 C
• UniProt Protein Name: Ubiquitin-conjugating enzyme E2 C
• Protein Family: Ubiquitin-conjugating enzyme
• UniProt Gene Name: UBE2C
• UniProt Synonym Gene Names: UBCH10

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]

Uniprot Description

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.