Rabbit anti-Human M6PR Polyclonal Antibody | anti-M6PR antibody

M6PR, CT (M6PR, MPR46, MPRD, Cation-dependent mannose-6-phosphate receptor, 46kD mannose 6-phosphate receptor) (MaxLight 405)

Cat. Num. AAA6317884

• Gene Names: M6PR; SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: M6PR; Polyclonal Antibody; CT (M6PR; MPR46; MPRD; Cation-dependent mannose-6-phosphate receptor; 46kD mannose 6-phosphate receptor) (MaxLight 405); 46kD mannose 6-phosphate receptor; anti-M6PR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-M6PR antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: M6PR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-268 amino acids from the C-terminal region of human M6PR.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(M6PR Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the M6PR antibody detected the M6PR protein (arrow).)

Flow Cytometry (FC/FACS)

(M6PR Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of M6PR Antibody (C-term)(AP2792b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-M6PR antibody

The protein encoded by this gene is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellular targeting of lysosomal enzymes and export of newly synthesized lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane.

Product Categories/Family for anti-M6PR antibody

Antibodies; Receptors

References

Bosker, F.J., et al. Mol. Psychiatry (2010). In press: Op den Brouw, M.L., et al. Virology 393(1):84-90(2009). Schimanski, L.M., et al. Blood Cells Mol. Dis. 43(2):180-193(2009). Rodriguez-Gabin, A.G., et al. Exp. Cell Res. 315(13):2215-2230(2009). Xaplanteri, P., et al. Eur. J. Immunol. 39(3):730-740(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4505061
• NCBI GeneID: 4074
• NCBI Accession #: NP_002346.1
• NCBI GenBank Nucleotide #: NM_002355.3
• UniProt Accession #: P20645; A8K528; D3DUV5
• Molecular Weight: 30,993 Da
• NCBI Official Full Name: cation-dependent mannose-6-phosphate receptor isoform 1
• NCBI Official Synonym Full Names: mannose-6-phosphate receptor, cation dependent
• NCBI Official Symbol: M6PR
• NCBI Official Synonym Symbols: SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
• NCBI Protein Information: cation-dependent mannose-6-phosphate receptor
• UniProt Protein Name: Cation-dependent mannose-6-phosphate receptor
• Protein Family: Cation-dependent mannose-6-phosphate receptor
• UniProt Gene Name: M6PR
• UniProt Synonym Gene Names: MPR46; MPRD; CD Man-6-P receptor; CD-MPR; MPR 46

NCBI Description

This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, May 2011]

Uniprot Description

Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.