Adenylyltransferase and sulfurtransferase UBA4 (UBA4) Recombinant Protein | UBA4 recombinant protein
Recombinant Ashbya gossypii Adenylyltransferase and sulfurtransferase UBA4 (UBA4)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Adenylyltransferase and sulfurtransferase UBA4 (UBA4); Recombinant Ashbya gossypii Adenylyltransferase and sulfurtransferase UBA4 (UBA4); Adenylyltransferase and sulfurtransferase UBA4; Ubiquitin-like protein activator 4; Including the following 2 domains:; Adenylyltransferase UBA4; EC=2.7.7.-; Sulfurtransferase UBA4; EC=2.8.1.-; UBA4 recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-443aa; full length protein
Sequence
MTGESLDGSL HALTIELDAL RRENANLKQQ LKEKDGACGE LPMSLEEFQR YGRQMIVGETGGLSGQVKLR SARVLIVGAG GLGCPALQYL AGAGIGHLGI VDNDVVEESN LHRQPLHDTSKVGLLKCDSA KEALSRLNPY CSIKTYPVRL SYANAFEIFP SWDLILDCTD SPMSRYLISDVAVNLGKTVV SGSGLGTEGQ LSIYNFENKG PCYRCFYPIP PRPGSVVSCQ SGGVLGPCIGVLGIMMAVEA LKILFGIYTL ENFKPFLMQY SGFPYQTLRM FKMRNRKQGC LCCGDNPTITKSTIESGHIK YEAFCGAINY DVLSKDERLS ASEFEANYWS QKERGFVCLD VRPRLHYEISHLPGTYNMTV KELDEMEGSI EELQKHIPVI TPDLDIVVLC RYGNDSRLAT RILKDKFKLRNVRDVKGGYF AYIDEINPSL PKY
Sequence Length
443
Species
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast) (Eremothecium gossypii)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,181 Da
NCBI Official Full Name
AER248Wp
NCBI Official Symbol
AGOS_AER248W
NCBI Protein Information
AER248Wp
UniProt Protein Name
Adenylyltransferase and sulfurtransferase UBA4
Protein Family
UniProt Gene Name
UBA4
UniProt Entry Name
UBA4_ASHGO
Uniprot Description
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Acts by mediating the C-terminal thiocarboxylation of sulfur carrier URM1. Its N-terminus first activates URM1 as acyl-adenylate (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 to form thiocarboxylation (-COSH) of its C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
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Product Notes
The UBA4 uba4 (Catalog #AAA1318218) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-443aa; full length protein. The amino acid sequence is listed below: MTGESLDGSL HALTIELDAL RRENANLKQQ LKEKDGACGE LPMSLEEFQR YGRQMIVGET GGLSGQVKLR SARVLIVGAG GLGCPALQYL AGAGIGHLGI VDNDVVEESN LHRQPLHDTS KVGLLKCDSA KEALSRLNPY CSIKTYPVRL SYANAFEIFP SWDLILDCTD SPMSRYLISD VAVNLGKTVV SGSGLGTEGQ LSIYNFENKG PCYRCFYPIP PRPGSVVSCQ SGGVLGPCIG VLGIMMAVEA LKILFGIYTL ENFKPFLMQY SGFPYQTLRM FKMRNRKQGC LCCGDNPTIT KSTIESGHIK YEAFCGAINY DVLSKDERLS ASEFEANYWS QKERGFVCLD VRPRLHYEIS HLPGTYNMTV KELDEMEGSI EELQKHIPVI TPDLDIVVLC RYGNDSRLAT RILKDKFKLR NVRDVKGGYF AYIDEINPSL PKY. It is sometimes possible for the material contained within the vial of "Adenylyltransferase and sulfurtransferase UBA4 (UBA4), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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