UBE1C Blocking Peptide | UBA3 blocking peptide

UBE1C Antibody (C-term) Blocking Peptide

• Gene Names: UBA3; NAE2; UBE1C; hUBA3
• Synonyms: UBE1C; UBE1C Antibody (C-term) Blocking Peptide; NEDD8-activating enzyme E1 catalytic subunit; 632-; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3; UBA3; UBA3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human UBE1C. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 463

• Tissue Location: Ubiquitously expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for UBA3 blocking peptide

Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.

NCBI and Uniprot Product Information

• NCBI GI #: 83305811
• NCBI GeneID: 9039
• UniProt Accession #: Q8TBC4; O76088; Q9NTU3; A6NLB5; A8K027
• Molecular Weight: 50,072 Da
• NCBI Official Full Name: NEDD8-activating enzyme E1 catalytic subunit
• NCBI Official Synonym Full Names: ubiquitin like modifier activating enzyme 3
• NCBI Official Symbol: UBA3
• NCBI Official Synonym Symbols: NAE2; UBE1C; hUBA3
• NCBI Protein Information: NEDD8-activating enzyme E1 catalytic subunit
• UniProt Protein Name: NEDD8-activating enzyme E1 catalytic subunit
• Protein Family: Ubiquitin-activating enzyme
• UniProt Gene Name: UBA3
• UniProt Synonym Gene Names: UBE1C; Ubiquitin-activating enzyme 3
• UniProt Entry Name: UBA3_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ligase; Cell cycle regulation; Nuclear receptor co-regulator; Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: cytosol; nucleus

Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity

Biological Process: protein modification process; protein neddylation; proteolysis