Monkey Ubiquitin ELISA Kit | UB elisa kit

Monkey Ubiquitin ELISA Kit

Cat. Num. AAA739549

• Gene Names: RPS27A; UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80
• Reactivity: Monkey
• Synonyms: Ubiquitin; Monkey Ubiquitin ELISA Kit; UB elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This UB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey UB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UB elisa kit

Principle of the Assay: UB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for UB. Standards or samples are then added to the microtiter plate wells and UB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of UB present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for UB are added to each well to "sandwich" the UB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain UB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for UB elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 37571
• NCBI GeneID: 6233
• UniProt Accession #: P62979; P02248; P02249; P02250; P14798; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888
• Molecular Weight: 17,965 Da
• NCBI Official Full Name: ubiquitin
• NCBI Official Synonym Full Names: ribosomal protein S27a
• NCBI Official Symbol: RPS27A
• NCBI Official Synonym Symbols: UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80
• NCBI Protein Information: ubiquitin-40S ribosomal protein S27a; ubiquitin C; ubiquitin-CEP80; epididymis luminal protein 112; ubiquitin and ribosomal protein S27a; ubiquitin carboxyl extension protein 80
• UniProt Protein Name: Ubiquitin-40S ribosomal protein S27a
• Protein Family: Ubiquitin
• UniProt Gene Name: RPS27A
• UniProt Synonym Gene Names: UBA80; UBCEP1
• UniProt Entry Name: RS27A_HUMAN

NCBI Description

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]

Uniprot Description

RPS27A: the gene (RPS27A) that encodes this protein is one of four that encode for ubiquitin: UBC, UBB, UBA52 and RPS27A. UBB and UBC genes code for a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The RPS27A gene product is cleaved into the following 2 chains: ubiquitin (amino acids 1-76) and the 40S ribosomal protein S27a (amino acids 77-156). Ubiquitin is a peptide 76 amino acids in length that can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Hundreds of ubiquitin ligases and hydrolases have been identified, implicating ubiquitin as a major regulatory element in many crucial cellular systems. It can be covalently bound to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. At the protein level, it is not possible to easily determine which of the four genes encoded a given ubiquitin chain.

Protein type: Ubiquitin-like modifier; Ribosomal

Chromosomal Location of Human Ortholog: 2p16

Cellular Component: nucleoplasm; small ribosomal subunit; membrane; cytoplasm; nucleolus; plasma membrane; endosome membrane; cytosol

Molecular Function: structural constituent of ribosome; metal ion binding

Biological Process: circadian rhythm; I-kappaB kinase/NF-kappaB cascade; SRP-dependent cotranslational protein targeting to membrane; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; endosome transport; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; regulation of apoptosis; toll-like receptor 5 signaling pathway; antigen processing and presentation of peptide antigen via MHC class I; transforming growth factor beta receptor signaling pathway; translational initiation; antigen processing and presentation of exogenous peptide antigen via MHC class I; JNK cascade; viral transcription; G2/M transition of mitotic cell cycle; toll-like receptor 4 signaling pathway; regulation of interferon type I production; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; Notch receptor processing; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; glucose metabolic process; virus assembly; toll-like receptor 2 signaling pathway; translational elongation; carbohydrate metabolic process; viral protein processing; mRNA catabolic process, nonsense-mediated decay; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of interferon type I production; negative regulation of apoptosis; G1/S transition of mitotic cell cycle; negative regulation of epidermal growth factor receptor signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; translation; apoptosis; pathogenesis; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; translational termination; toll-like receptor 10 signaling pathway; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of interferon type I production; transmembrane transport; epidermal growth factor receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; DNA repair; MyD88-dependent toll-like receptor signaling pathway; cellular protein metabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; toll-like receptor signaling pathway; innate immune response; gene expression; mitotic cell cycle; negative regulation of transforming growth factor beta receptor signaling pathway