Typical Testing Data/Standard Curve (for reference only)
Rat Myosin Light Chain 3 ELISA Kit | MYL3 elisa kit
Rat Myosin Light Chain 3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat MYL1 antibody. MYL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat MYL1 Antibody is added and binds to MYL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat MYL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
Uniprot Description
MYL3: Regulatory light chain of myosin. Does not bind calcium. Defects in MYL3 are the cause of familial hypertrophic cardiomyopathy type 8 (CMH8). Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
Protein type: Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 3p21.3-p21.2
Cellular Component: I band; sarcomere; muscle myosin complex; cytosol; A band
Molecular Function: actin monomer binding; structural constituent of muscle; motor activity; myosin II heavy chain binding; calcium ion binding
Biological Process: skeletal muscle development; positive regulation of ATPase activity; metabolic process; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; regulation of the force of heart contraction; cardiac muscle contraction; muscle filament sliding
Disease: Cardiomyopathy, Familial Hypertrophic, 8
Research Articles on MYL3
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Product Notes
The Rat MYL3 myl3 (Catalog #AAA1603462) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603462 ELISA Kit recognizes Rat MYL3. It is sometimes possible for the material contained within the vial of "Myosin Light Chain 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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