Human Ubiquitin ELISA Kit | Ub elisa kit

Human Ubiquitin (Ub) ELISA Kit

Cat. Num. AAA2031856

• Gene Names: UBC; HMG20
• Reactivity: Human
• Synonyms: Ubiquitin; Human Ubiquitin (Ub) ELISA Kit; Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B; Ub elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin (Ub).; No significant cross-reactivity or interference between Ubiquitin (Ub) and analogues was observed.
• Sequence Length: 685

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.42ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin (Ub) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin (Ub) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for Ub elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin (Ub). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin (Ub). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin (Ub), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin (Ub) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Ub elisa kit

Reproductive science; Neuroscience; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 340068
• NCBI GeneID: 7316
• Molecular Weight: 77,039 Da
• NCBI Official Full Name: ubiquitin
• NCBI Official Synonym Full Names: ubiquitin C
• NCBI Official Symbol: UBC
• NCBI Official Synonym Symbols: HMG20
• NCBI Protein Information: polyubiquitin-C
• UniProt Protein Name: Polyubiquitin-C
• Protein Family: Ubiquitin
• UniProt Gene Name: UBC
• UniProt Entry Name: UBC_HUMAN

NCBI Description

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010]

Uniprot Description

UBC: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Belongs to the ubiquitin family.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: cytosol; endosome membrane; extracellular space; myelin sheath; nucleoplasm; nucleus; plasma membrane

Molecular Function: protease binding; protein binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; activation of NF-kappaB transcription factor; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; apoptosis; axon guidance; bypass DNA synthesis; carbohydrate metabolic process; cell surface receptor linked signal transduction; circadian rhythm; cytokine and chemokine mediated signaling pathway; DNA damage response, detection of DNA damage; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; endosome transport; epidermal growth factor receptor signaling pathway; error-prone postreplication DNA repair; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; gene expression; glucose metabolic process; glycogen biosynthetic process; I-kappaB kinase/NF-kappaB cascade; innate immune response; insulin receptor signaling pathway; JNK cascade; macroautophagy; macromitophagy; MAPKKK cascade; mitotic cell cycle; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of interferon type I production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; nerve growth factor receptor signaling pathway; Notch receptor processing; Notch signaling pathway; nucleotide-excision repair; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; positive regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; programmed cell death; protein polyubiquitination; Ras protein signal transduction; regulation of apoptosis; regulation of interferon type I production; regulation of mRNA stability; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transcription-coupled nucleotide-excision repair; transforming growth factor beta receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral infectious cycle; viral protein processing; viral reproduction; virus assembly