Human Urotensin 2 ELISA Kit | U-II elisa kit

Human Urotensin 2 ELISA Kit

Cat. Num. AAA762653

• Gene Names: UTS2; UII; U-II; UCN2; PRO1068
• Reactivity: Human
• Synonyms: Urotensin 2; Human Urotensin 2 ELISA Kit; U-II (Urotensin 2)/UII/Urotensin II; U-II elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of U-II. No significant cross-reactivity or interference between U-II and analogues was observed.
• Sequence Length: 124

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for U-II elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5803209
• NCBI GeneID: 10911
• NCBI Accession #: NP_006777.1
• NCBI GenBank Nucleotide #: NM_006786.3
• Molecular Weight: 16,029 Da
• NCBI Official Full Name: urotensin-2 isoform b preproprotein
• NCBI Official Synonym Full Names: urotensin 2
• NCBI Official Symbol: UTS2
• NCBI Official Synonym Symbols: UII; U-II; UCN2; PRO1068
• NCBI Protein Information: urotensin-2
• UniProt Protein Name: Urotensin-2
• UniProt Gene Name: UTS2
• UniProt Synonym Gene Names: U-II; UII
• UniProt Entry Name: UTS2_HUMAN

NCBI Description

This gene encodes a mature peptide that is an active cyclic heptapeptide absolutely conserved from lamprey to human. The active peptide acts as a vasoconstrictor and is expressed only in brain tissue. Despite the gene family name similarity, this gene is not homologous to urocortin, a member of the sauvagine/corticotropin-releasing factor/urotensin I family. Most of the proprotein is cleaved to make the mature peptide. Transcript variants encoding different preproprotein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UTS2: Highly potent vasoconstrictor. Belongs to the urotensin-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cytosol; extracellular region; extracellular space

Molecular Function: hormone activity; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; muscle contraction; negative regulation of blood pressure; negative regulation of glomerular filtration; negative regulation of heart rate; negative regulation of insulin secretion; positive regulation of angiogenesis; positive regulation of blood pressure; positive regulation of cell differentiation; positive regulation of circadian sleep/wake cycle, REM sleep; positive regulation of collagen biosynthetic process; positive regulation of fibroblast proliferation; positive regulation of heart rate; positive regulation of synaptic transmission, cholinergic; positive regulation of vasodilation; regulation of blood pressure; response to drug; response to hypoxia; response to testosterone stimulus; synaptic transmission