Western Blot (WB)
(TSFM Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TSFM antibody detected the TSFM protein (arrow).)
Rabbit TSFM Polyclonal Antibody | anti-TSFM antibody
TSFM Antibody (C-term)
Western Blot (WB)
(TSFM Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TSFM antibody detected the TSFM protein (arrow).)
Western Blot (WB)
(TSFM Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TSFM antibody detected the TSFM protein (arrow).)
factor. The encoded protein is an enzyme that catalyzes the
exchange of guanine nucleotides on the translation elongation
factor Tu during the elongation step of mitchondrial protein
translation. Mutations in this gene are associated with combined
oxidative phosphorylation deficiency-3 syndrome. Alternate splicing
results in multiple transcript variants.
Smeitink, J.A., et al. Am. J. Hum. Genet. 79(5):869-877(2006)
Antonicka, H., et al. Hum. Mol. Genet. 15(11):1835-1846(2006)
Vernon, J.L., et al. Cytogenet. Cell Genet. 89 (3-4), 145-146 (2000) :
Xin, H., et al. J. Biol. Chem. 270(29):17243-17249(1995)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]
Uniprot Description
EFT5: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome. Defects in TSFM are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD3 symptoms include severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Patients show a severe defect in mitochondrial translation leading to a failure to assemble adequate amounts of three of the oxidative phosphorylation complexes. Belongs to the EF-Ts family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Translation elongation; Translation
Chromosomal Location of Human Ortholog: 12q14.1
Cellular Component: nucleoplasm; mitochondrion; nucleus
Molecular Function: translation elongation factor activity
Biological Process: translational elongation; mitochondrial translation; organelle organization and biogenesis; regulation of RNA elongation
Disease: Combined Oxidative Phosphorylation Deficiency 3
Research Articles on TSFM
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Product Notes
The TSFM tsfm (Catalog #AAA9211916) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 253-282. The TSFM Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's TSFM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the TSFM tsfm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSFM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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