TXNDC17 recombinant protein

TXNDC17 Protein, Human, Recombinant

Cat. Num. AAA8120773

• Gene Names: TXNDC17; TRP14; TXNL5
• Purity: >97% as determined by SDS-PAGE
• Synonyms: TXNDC17; TXNDC17 Protein; Human; Recombinant; Human TXNDC17/TRP14/TXNL5 Protein; TRP14 Protein; TXNL5 Protein; thioredoxin domain containing 17; TXNDC17 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp123

• Species: Human
• Predicted N Terminal: Met 1
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human TXNDC17 (Q9BRA2)(Met 1-Asp 123) was expressed and purified, with an initial Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TXNDC17 recombinant protein

Background: Thioredoxin-related protein of 14kDa (TRP14, also called TXNDC17 for thioredoxin domain containing 17, or TXNL5 for thioredoxin-like 5) is an evolutionarily well-conserved member of the thioredoxin (Trx)-fold protein family that lacks activity with classical Trx1 substrates. TXNDC17 is a novel 14-kDa disulfide reductase of the TXN (thioredoxin) family. TXNDC17 is involved in the TNF (tumor necrosis factor) signaling pathway. Moreover, TXNDC17 plays an important role in Taxol resistance via enhancing autophagy in human colorectal cancer cells. And TXNDC17 may become a potential target of colorectal cancer therapeutics.

References

Holmgren A. (1985) Thioredoxin. Annu Rev Biochem. 54: 237-71.Holmgren A. (1995) Thioredoxin structure and mechanism: conformational changes on oxidation of the active-site sulfhydryls to a disulfide. Structure. 3 (3): 239-43.Martin JL. (1995) Thioredoxin--a fold for all reasons. Structure. 3 (3): 245-50.

NCBI and Uniprot Product Information

• NCBI GI #: 14249348
• NCBI GeneID: 84817
• NCBI Accession #: NP_116120.1
• NCBI GenBank Nucleotide #: NM_032731.3
• UniProt Accession #: Q9BRA2; A8K7E8
• Molecular Weight: 13,941 Da
• NCBI Official Full Name: thioredoxin domain-containing protein 17
• NCBI Official Synonym Full Names: thioredoxin domain containing 17
• NCBI Official Symbol: TXNDC17
• NCBI Official Synonym Symbols: TRP14; TXNL5
• NCBI Protein Information: thioredoxin domain-containing protein 17; 14 kDa thioredoxin-related protein; protein 42-9-9; thioredoxin (Trx)-related protein, 14 kDa; thioredoxin-like 5; thioredoxin-like protein 5
• UniProt Protein Name: Thioredoxin domain-containing protein 17
• UniProt Gene Name: TXNDC17
• UniProt Synonym Gene Names: TXNL5; TRP14
• UniProt Entry Name: TXD17_HUMAN

Uniprot Description

TXNL5: Disulfide reductase. May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions. Modulates TNF-alpha signaling and NF-kappa-B activation. Has peroxidase activity and may contribute to the elimination of cellular hydrogen peroxide. Belongs to the thioredoxin family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytosol

Molecular Function: protein-disulfide reductase activity; protein binding; peroxidase activity; electron carrier activity

Biological Process: tumor necrosis factor-mediated signaling pathway