Rabbit anti-Human TOPBP1 Polyclonal Antibody | anti-TOPBP1 antibody

TOPBP1 (S1159) (TOPBP1, KIAA0259, DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, DNA topoisomerase II-binding protein 1) (Azide free) (HRP)

Cat. Num. AAA6357388

• Gene Names: TOPBP1; TOP2BP1
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TOPBP1; Polyclonal Antibody; TOPBP1 (S1159) (TOPBP1; KIAA0259; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1) (Azide free) (HRP); DNA topoisomerase II-binding protein 1; anti-TOPBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TOPBP1 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-TOPBP1-S1159 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding S1159 of human TOPBP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-TOPBP1-S1159 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-TOPBP1 antibody

This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.

Product Categories/Family for anti-TOPBP1 antibody

Antibodies; Topoisomerases

References

Huo, Y.G., et al. Biochem. Biophys. Res. Commun. 401(3):401-405(2010). Blackford, A.N., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12251-12256(2010). Takeishi, Y., et al. Genes Cells 15(7):761-771(2010). Gong, Z., et al. Mol. Cell 37(3):438-446(2010). Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 194440660
• NCBI GeneID: 11073
• NCBI Accession #: NP_008958.2
• NCBI GenBank Nucleotide #: NM_007027.3
• UniProt Accession #: Q92547; Q7LGC1; Q9UEB9; B7Z7W8
• Molecular Weight: 170,679 Da
• NCBI Official Full Name: DNA topoisomerase 2-binding protein 1
• NCBI Official Synonym Full Names: topoisomerase (DNA) II binding protein 1
• NCBI Official Symbol: TOPBP1
• NCBI Official Synonym Symbols: TOP2BP1
• NCBI Protein Information: DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1
• UniProt Protein Name: DNA topoisomerase 2-binding protein 1
• Protein Family: DNA topoisomerase 2-binding protein
• UniProt Gene Name: TOPBP1
• UniProt Synonym Gene Names: KIAA0259; TopBP1
• UniProt Entry Name: TOPB1_HUMAN

NCBI Description

This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]

Uniprot Description

TOPBP1: a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: male germ cell nucleus; spindle pole; Golgi apparatus; centrosome; PML body; condensed nuclear chromosome; cytoplasm; chromosome; nucleus; actin cytoskeleton

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; DNA binding

Biological Process: response to ionizing radiation; DNA repair; DNA metabolic process; response to DNA damage stimulus