Typical Testing Data/Standard Curve (for reference only)
Chicken beta-tubulin ELISA Kit | TUBB elisa kit
Chicken beta-tubulin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This TUBB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken TUBB. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY TUBB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TUBB antibody and an TUBB-HRP conjugate. The assay sample and buffer are incubated together with TUBB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of theenzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TUBB concentration since TUBB from samples and TUBB-HRP conjugate compete for the anti-TUBB antibody binding site. Since the number of sites is limited, as more sites are occupied by TUBB from the sample, fewer sites are left to bind TUBB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TUBB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
Uniprot Description
TUBB2A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: microtubule; cytoplasm; nucleus; vesicle
Molecular Function: GTPase activity; GTP binding; structural constituent of cytoskeleton
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; transmembrane transport; microtubule-based process
Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 5
Research Articles on TUBB
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Product Notes
The Chicken TUBB tubb2a (Catalog #AAA737854) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737854 ELISA Kit recognizes Chicken TUBB. It is sometimes possible for the material contained within the vial of "beta-tubulin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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