Thyroid Stimulating Hormone alpha, beta Recombinant Protein | TSHB recombinant protein
Thyroid Stimulating Hormone alpha, beta, Recombinant, Human (TSHa, TSHb) (BSA Free)
Gene Names
TSHB; TSH-B; TSH-BETA
Purity
Highly Purified90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Synonyms
Thyroid Stimulating Hormone alpha; beta; Recombinant; Human (TSHa; TSHb) (BSA Free); TSHB recombinant protein
Host
Recombinant Human
Purity/Purification
Highly Purified
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.
Endotoxin Level
1EU/1ug.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for TSHB recombinant protein
Thyroid-stimulating hormone (TSH or thyrotropin), produced in human pituitary glands, has a molecular mass of 25kD. An Important indicator of thyroid function, TSH is used to monitor thyroid associated diseases. Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release.
The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH. When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The alpha subunit is identical to that of human chorionic gonadotropin (HCG), luteinizing hormone (LH), follicle-stimulating hormone (FSH). The beta subunit is unique to TSH, and therefore determines its function.
TSH is a member of the cystine knot growth factor superfamily. It is a heterodimer of a 15kD unique subunit, TSH beta, with a 14kD alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Beta subunits of the four glycoprotein hormones share a 37-43% amino acid identity. Mature human TSH beta shares a 92%, 90%, 90%, 89%, 89%, 89%, and 88% aa identity with dog, rat, horse, mouse, cow, pig, and cat TSH b, respectively. Mature human CGa shares a 69%-73% aa identity with dog, rabbit, rat, mouse, cow, sheep, pig, cat and horse CGa. Each subunit forms a cystine knot structure with three disulfide bridges. A loop of the TSH beta subunit, termed a 'seat-belt', wraps around the CGa subunit to stabilize non-covalent association of the subunits, and also confers receptor selectivity.
Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but longer halflife. Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity. The hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland. TSH travels to thyroid TSHR to stimulate production of thyroxine (T4). In the tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production. Studies in mouse identify bone marrow as a secondary site of TSH production. In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling. Bone marrow cells that produce TSH may also circulate to the thyroid and appear to modulate thyroid hormone activity in times of immunological stress.
DNA sequences encoding human TSH-a (residues 1-116; Accession # P01215) and human TSH-b (residues 1-138; Accession # P01222) were co-expressed in Chinese Hamster Ovary cells.
Calculated molecular masses of 10.2 and 13.5kD, respectively. The recombinant protein is a non-covalent linked heterodimer. As a result of glycosylation, the recombinant human TSH a/b migrates at ~ 15-29kD on SDS-PAGE under reducing conditions.
Activity; Measured by its ability to induce cAMP accumulation in human TSH receptor transfected HEK293 cells.
The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH. When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The alpha subunit is identical to that of human chorionic gonadotropin (HCG), luteinizing hormone (LH), follicle-stimulating hormone (FSH). The beta subunit is unique to TSH, and therefore determines its function.
TSH is a member of the cystine knot growth factor superfamily. It is a heterodimer of a 15kD unique subunit, TSH beta, with a 14kD alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Beta subunits of the four glycoprotein hormones share a 37-43% amino acid identity. Mature human TSH beta shares a 92%, 90%, 90%, 89%, 89%, 89%, and 88% aa identity with dog, rat, horse, mouse, cow, pig, and cat TSH b, respectively. Mature human CGa shares a 69%-73% aa identity with dog, rabbit, rat, mouse, cow, sheep, pig, cat and horse CGa. Each subunit forms a cystine knot structure with three disulfide bridges. A loop of the TSH beta subunit, termed a 'seat-belt', wraps around the CGa subunit to stabilize non-covalent association of the subunits, and also confers receptor selectivity.
Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but longer halflife. Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity. The hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland. TSH travels to thyroid TSHR to stimulate production of thyroxine (T4). In the tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production. Studies in mouse identify bone marrow as a secondary site of TSH production. In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling. Bone marrow cells that produce TSH may also circulate to the thyroid and appear to modulate thyroid hormone activity in times of immunological stress.
DNA sequences encoding human TSH-a (residues 1-116; Accession # P01215) and human TSH-b (residues 1-138; Accession # P01222) were co-expressed in Chinese Hamster Ovary cells.
Calculated molecular masses of 10.2 and 13.5kD, respectively. The recombinant protein is a non-covalent linked heterodimer. As a result of glycosylation, the recombinant human TSH a/b migrates at ~ 15-29kD on SDS-PAGE under reducing conditions.
Activity; Measured by its ability to induce cAMP accumulation in human TSH receptor transfected HEK293 cells.
Product Categories/Family for TSHB recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,639 Da
NCBI Official Full Name
thyrotropin subunit beta isoform 1
NCBI Official Synonym Full Names
thyroid stimulating hormone, beta
NCBI Official Symbol
TSHB
NCBI Official Synonym Symbols
TSH-B; TSH-BETA
NCBI Protein Information
thyrotropin subunit beta; thyrotropin beta chain
UniProt Protein Name
Thyrotropin subunit beta
Protein Family
UniProt Gene Name
TSHB
UniProt Synonym Gene Names
TSH-B; TSH-beta
UniProt Entry Name
TSHB_HUMAN
NCBI Description
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Uniprot Description
TSHB: Indispensable for the control of thyroid structure and metabolism. Belongs to the glycoprotein hormones subunit beta family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13
Cellular Component: extracellular region
Molecular Function: hormone activity
Biological Process: G-protein coupled receptor protein signaling pathway; anatomical structure morphogenesis; cellular protein metabolic process; cell-cell signaling; response to estrogen stimulus; peptide hormone processing; response to calcium ion; response to vitamin A
Disease: Hypothyroidism, Congenital, Nongoitrous, 4
Research Articles on TSHB
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Product Notes
The TSHB tshb (Catalog #AAA636633) is a Recombinant Protein produced from Recombinant Human and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thyroid Stimulating Hormone alpha, beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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