Rat Thioredoxin Reductase 1 ELISA Kit | TrxR1 elisa kit

Rat Thioredoxin Reductase 1 ELISA Kit

Cat. Num. AAA760458

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Reactivity: Rat
• Synonyms: Thioredoxin Reductase 1; Rat Thioredoxin Reductase 1 ELISA Kit; TrxR1; TrxR1 elisa kit

• Reactivity: Rat
• Sequence Length: 647

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TrxR1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat TrxR1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat TrxR1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat TrxR1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat TrxR1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 37542493
• NCBI GeneID: 7296
• Molecular Weight: 50,873 Da
• NCBI Official Full Name: thioredoxin reductase 1
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; EC 1.8.1.9; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: cytosol; mitochondrion; nucleus

Molecular Function: electron carrier activity; FAD binding; protein binding; protein disulfide oxidoreductase activity; thioredoxin-disulfide reductase activity

Biological Process: cell proliferation; cell redox homeostasis; cellular lipid metabolic process; gene expression; mesoderm formation; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; response to reactive oxygen species; selenium metabolic process; signal transduction; transcription initiation from RNA polymerase II promoter