Rat Complement Factor D (CFD) ELISA Kit | CFD elisa kit

Rat Complement Factor D (CFD) ELISA Kit

Cat. Num. AAA2022239

• Gene Names: CFD; DF; ADN; PFD; ADIPSIN
• Reactivity: Rat
• Synonyms: Complement Factor D (CFD); Rat Complement Factor D (CFD) ELISA Kit; CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator; CFD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD).; No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.
• Sequence Length: 253

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.27ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor D (CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CFD elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 42544239
• NCBI GeneID: 1675
• NCBI Accession #: NP_001919.2
• NCBI GenBank Nucleotide #: NM_001928.2
• UniProt Accession #: P00746; Q5U5S1; Q86VJ5; Q8N4E0; Q8WZB4; B4DV76
• Molecular Weight: 27,033 Da
• NCBI Official Full Name: complement factor D preproprotein
• NCBI Official Synonym Full Names: complement factor D (adipsin)
• NCBI Official Symbol: CFD
• NCBI Official Synonym Symbols: DF; ADN; PFD; ADIPSIN
• NCBI Protein Information: complement factor D
• UniProt Protein Name: Complement factor D
• Protein Family: Complement factor
• UniProt Gene Name: CFD
• UniProt Synonym Gene Names: DF; PFD
• UniProt Entry Name: CFAD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the trypsin family of peptidases. The encoded protein is a component of the alternative complement pathway best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. [provided by RefSeq, Jul 2008]

Uniprot Description

CFD: Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. Defects in CFD are the cause of complement factor D deficiency (CFDD). CFDD is an immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted; EC 3.4.21.46; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: platelet activation; platelet degranulation; complement activation, alternative pathway; innate immune response; blood coagulation; proteolysis; complement activation

Disease: Complement Factor D Deficiency