Rat Thioredoxin Reductase 1 ELISA Kit | TrxR1 elisa kit

Rat Thioredoxin Reductase 1 (TrxR1) ELISA Kit

Cat. Num. AAA2033670

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Reactivity: Rat
• Synonyms: Thioredoxin Reductase 1; Rat Thioredoxin Reductase 1 (TrxR1) ELISA Kit; TXNRD1; TR1; GRIM12; KDRF; TRXR-1; TXNR; Gene associated with retinoic and interferon-induced mortality 12 protein; KM-102-derived reductase-like factor; TrxR1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thioredoxin Reductase 1 (TrxR1).; No significant cross-reactivity or interference between Thioredoxin Reductase 1 (TrxR1) and analogues was observed.
• Sequence Length: 647

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TrxR1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin Reductase 1 (TrxR1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve

Related Product Information for TrxR1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Reductase 1 (TrxR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin Reductase 1 (TrxR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Reductase 1 (TrxR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin Reductase 1 (TrxR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TrxR1 elisa kit

Signal transduction; Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 37542493
• NCBI GeneID: 7296
• Molecular Weight: 50,873 Da
• NCBI Official Full Name: thioredoxin reductase 1
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• Protein Family: Thioredoxin reductase
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Nuclear receptor co-regulator; Oxidoreductase; EC 1.8.1.9; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: cytosol; mitochondrion; nucleus

Molecular Function: electron carrier activity; FAD binding; protein binding; protein disulfide oxidoreductase activity; thioredoxin-disulfide reductase activity

Biological Process: cell proliferation; cell redox homeostasis; cellular lipid metabolic process; gene expression; mesoderm formation; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; response to reactive oxygen species; selenium metabolic process; signal transduction; transcription initiation from RNA polymerase II promoter